Lena Mathew Email & Phone Number

San Ramon, Ca, Us

San Ramon, Ca, Us

Seattle, Washington, United States

Seattle, Washington, United States

Greater Seattle Area

Greater Seattle Area

• Oversees research and personnel functions and ensures CLIA compliance in the laboratory• Manage regulatory documentation and development activities• Maintain QC, QA and proficiency test documentation• Train and supervise NCGL researchers as well as new laboratory personnel• Lead development of laboratory workflows and testing • Supports bioinformatics team with LIMS development • Continues Research Scientist III duties stated below

Greater Seattle Area

• Provide clinical-grade whole exome, whole genome and targeted gene sequencing of patient and research subject-derived samples in a CAP certified lab• Isolate DNA from human blood, saliva, tissue and FFPE slides using automated and manual techniques• Use LIMs to perform data entry, editing, quality control and quality assurance• Perform primary data quality evaluation and review variant results• Establish research protocols; develop laboratory workflows and testing• Establish and follow laboratory SOPs for processing, test analyses, reporting and maintaining records of subject test results

San Jose, Ca

•Performed prenatal, diagnostic and screening genetic tests in a CLIA regulated Regional Genetics Lab •Performed a variety of molecular biology techniques including: -DNA extraction from blood, direct and cultured amnios, tissues, CVS samples and FFPE slides. -RNA extraction from blood and bone marrow -QPCR and RT PCR -DNA hybridization assays such as Southern Blot and Reverse Dot Blots -DNA labeling for aCGH -Run agarose and acrylamide gels • Proficient in automated instrumentation including: Qiagen MDx, Bio-robot, Qiacube and Qiagility, Biomek FX, ABI 3130 and 3500xl Genetic Analyzers, Luminex, Third Wave Technology, ViiA7• Fully trained and user of the Ion Torrent and part of the Next Gen Sequencing group• Performed assays on genetic tests including: Fragile X, Myotonic Dystrophy, Alpha thalassemia, CF, Beta Thal, Sanger sequencing, EGFR and EGFR Pathway, STR(MCC), Tay Sachs, Canavans, Familial Dysautonomia, BCR-abl, Prader Willi/Angelman's, Huntington's, UGT1A1, JAK2• Assisted in the investigation and evaluation of new protocols and techniques • Member of the Contamination Prevention work group and Unit Based Team -Found ways to re-arrange the lab in order to separate Pre and Post-PCR -Identified areas where contamination may possibly be introduced -Performed projects that increase efficiency, cost-savings and tech time

Walnut Creek, Ca

• Provided technical support for DNA sequencing tasks in a high-throughput sequencing environment • Performed daily tasks on the ABI 3730xl sequencer including maintenance, loading and troubleshooting errors• Worked on editing and organizing audits of protocols• Scheduled tasks for the capillaries group as well as trained new employees• Was in charge of ordering reagents and performed QC on new shipments• Conducted monthly conference calls with vendors • Performed various quality control checkpoints for the production line including running gels and stamping glycerol stocks• Identified and wrote reports on poor performing instruments as well as poor performing plates/batches

• Familiar with general USDA procedures and protocols• Performed various tasks including tending rice research plots, collecting rice tissue samples• Isolated DNA, performed PCR on rice DNA• Identified and characterized genes controlling traits that are agronomically important and utilize this information to improve temperate rice production• Assisted research on finding mutated forms of rice with low phytic acid which will lead to less environmental decay