Luca Rosa, Ph.D. Email & Phone Number

London, Gb

London Area, United Kingdom

London, England, United Kingdom

London, England, United Kingdom

London, United Kingdom

The aim of my PhD project was to develop an architecture capable of combining different species of biosensors to perform a multiplexed computation of multiple inputs and allowing an easy readout through pattern formation. The development of a proof-of-principle system was aided with a reaction-diffusion mathematical model and consists of two strains arranged in a grid structure: the “sender” strain integrates the biosensor system and produces N-Acyl homoserine lactone (AHL) which diffuses in… Show more The aim of my PhD project was to develop an architecture capable of combining different species of biosensors to perform a multiplexed computation of multiple inputs and allowing an easy readout through pattern formation. The development of a proof-of-principle system was aided with a reaction-diffusion mathematical model and consists of two strains arranged in a grid structure: the “sender” strain integrates the biosensor system and produces N-Acyl homoserine lactone (AHL) which diffuses in the agar, and a “receiver” strain that expresses a reporter protein in response to AHL molecules. Show less

I was responsible for marking students' tutorial work and then running a one hour tutorial with the class

Cambridge, England, United Kingdom

During my time at Microsoft I have characterised a band detect genetic circuit. The system responds to a gradient of diffusing AHL-C6, an intercellular signalling molecule, and produces AHL-C12. When the concentration of AHL-C12 is greater than the concentration of AHL-C6 the system expresses a yellow fluorescent protein (YFP). Using a mathematical model developed in the group I have simulated different experimental conditions to create spatial logic gates. The experimental set-up consists in a… Show more During my time at Microsoft I have characterised a band detect genetic circuit. The system responds to a gradient of diffusing AHL-C6, an intercellular signalling molecule, and produces AHL-C12. When the concentration of AHL-C12 is greater than the concentration of AHL-C6 the system expresses a yellow fluorescent protein (YFP). Using a mathematical model developed in the group I have simulated different experimental conditions to create spatial logic gates. The experimental set-up consists in a grid of bacteria with the AHL-C6 inputs on both sides. I have successfully managed to experimentally recreate an OR and XOR spatial logic gates where the output is YFP expression. Show less

Institue Of Child Health - London

During my time at the Institute of Child Health I worked on three different approaches to find a potential treatment for cystic fibrosis (CF). CF is due to a mutation in the CFTR gene, a chloride channel involved in the regulation of the epithelial sodium channel (ENaC). Loss of CFTR is thought to lessen the inhibition of ENaC leading to an altered ion homoeostasis. In my research project the aim is to functionally disrupt ENaC both with siRNA and with the Crispr/Cas9 technology using our… Show more During my time at the Institute of Child Health I worked on three different approaches to find a potential treatment for cystic fibrosis (CF). CF is due to a mutation in the CFTR gene, a chloride channel involved in the regulation of the epithelial sodium channel (ENaC). Loss of CFTR is thought to lessen the inhibition of ENaC leading to an altered ion homoeostasis. In my research project the aim is to functionally disrupt ENaC both with siRNA and with the Crispr/Cas9 technology using our Receptor Targeted Nanocomplex (RTN) as a system for delivery. The third approach that I used is to deliver the CFTR mRNA chemically modified in order to being more stable and less immunogenic. Show less

Bergamo, Italia

Doctor Of Philosophy (Ph.D.), Computational Systems And Synthetic Biology

Master'S Degree - Biology, Genetics, 110 Cum Laude

Bachelor'S Degree - Biotechnology