Luís Marques Email & Phone Number

Lisbon, Portugal

Lisbon, Portugal

Lisbon, Portugal

- Process development of bacteriophages and recombinant proteins- Upstream process design at 10 -50 L scale bioreactors- Downstream process design and development - GMP tech transfer- Reports writing - Project management

Oeiras, Portugal

- Process development scientist at Sanofi Satellite Lab- Downstream process development and optimization- Responsible for the bioprocess development of a vaccine within a multinational integrated consortium for Trypanosoma cruzi infection.- Clients' meetings management- Reports writing- project management

Stevenage, United Kingdom

Developing downstream processing strategies for viral vector purification - AAVs.- Focused in the development of gene therapy medicines for the treatment of eye diseases, linked to an unbalanced complement system, such as AMD (age-related macular degeneration).

Epsrc Centre For Innovative Manufacturing In Regenerative Medicine

Development of a cell therapy for Alzheimer's disease, exploring the role of microglia cells in the disease pathology.- Oral and poster scientific presentations in international conferences.- Scientific writing.- Project management and student’s co-supervision.- Extensive knowledge in Neurosciences, Cell therapy, Alzheimer's disease and Regenerative medicine- Large data set analysis.- Cell culture.- Image analysis. - qRT-PCR.- Flow cytometry.- ELISA.- Immunostaining.- Cell senescence research. - Phagocytosis assay.- Hierarchical clustering analysis.- Microglia cells co-culture.- Multiple sites team work collaborations: Nottingham University, Fraunhofer IZI (Germany), Keele University and Birmingham University.

Leipzig, Saxony, Germany

Microglia cell research, Alzheimer's disease, AxioScan microscopy, cell therapy, pre-clinical studies.

Keele, England, United Kingdom

Defining optimal growth environments for controlled differentiation of embryonic stem cells into cartilage;Hydrostatic bioreactor research on human embryonic stem cells differentiation into cartilage.

Nottingham, England, United Kingdom

3D Bioprinting of Vascularised Bone Tissues;

Oeiras, Lisbon, Portugal

Bioprocessing of biopharmaceuticals, monoclonal antibodies and recombinant proteins.Development and optimization projects;Production projects;Upstream Processing - mammalian cell culture, bacteria and insect cells as biological systems, from lab scale to Pilot scale (up to 300 L).Downstream Processing – Purification processes, chromatography (affinity, IEX, SEC, RP), dead-end filtration, TFF, dialysis, dyafiltration, product concentration.Endotoxin removal and quantification.Quality Control methods - SDS-PAGE, Western Blot, ELISA, spectrophotometry, protein quantification methods (BCA, bradford).SOP writing, organisation under tight deadlines to meet agreed milestones, planning and multi-tasking capacity.Teamwork spirit.

Oeiras, Lisbon, Portugal

Master Thesis performed at Instituto de Tecnologia Química e Biológica (ITQB). "Extraction of natural antioxidants from coffee plant and its incorporation in model pharmaceutical systems".Extraction, identification and analysis of polyphenols present in coffee plant leaves (oxidative stress and "anti-aging" compounds);HPLC-RP;Characterization and creation of a non-ionic microemulsion system for the delivery of an antioxidant from the coffee leaves;Dynamic Light Scattering (DLS);DPPH Antioxidant assay.Data analysis, literature review, statistical analysis, oral and poster scientific presentations.

Study the effect of radiation on DNA repair enzymes. Produce an overexpression system in E. coli for production of recombinant proteins with Fe-S centers. Purification of recombinant proteins and biochemical analysis.HPLC, preparative chromatography, affinity, size exclusion, ion exchange.UV/Vis spectrophotometry and fluorescence spectroscopy.SDS-PAGE and agarose gel.Scientific data anaysis.

Doctor Of Philosophy - Phd, Regenerative Medicine - Neurosciences

Development of cell therapies for Alzheimer's disease.

Specialization, Medical Affairs And Medical Marketing

Clinical vs. Medical Affairs Operations; Medical Plan and Roadmap to market access; Medical Affairs in the Business Strategy; Product.

Master'S Degree, Biotechnology, 17

Graduation, Biochemistry