As an Academic Tutor, I specialize in Science, Chemistry, Physics, and Mathematics up to Multi-Variable Calculus, and Discrete Math, for grades 8-12 and college students. My role involves:Providing personalized tutoring to help students understand complex concepts and achieve their academic goals.Working with students in the Supplemental Education Services “No Child Left Behind” program to enhance their confidence and academic performance.Supporting special needs children to ensure their academic success through tailored teaching strategies and dedicated support.My approach focuses on fostering a positive learning environment and developing strategies that cater to individual learning needs, helping students excel in their studies.

I ensured the smooth execution of analytical validation study activities from start-up to closeout, maintaining strict compliance with protocols and objectives. My work was integral to the successful validation and commercial launch of the next-generation liquid biopsy-based Guardant360® assays.Led the planning and execution of validation studies, coordinating cross-functional efforts to ensure readiness for commercial launch.Designed and managed sample selection for Concordance and Accuracy studies.Participated in the design and planning of Limit of Detection and Limit of Blank studies.Analyzed and presented pre-Analytical Validation (pre-AV) data read-outs to guide decision-making.Played a critical role in ensuring the quality execution of clinical protocols in accordance with SOPs, ICH/GCP guidelines, and corporate objectives.Contributed to the development of key study documents and supported various study-wide activities to maintain quality and adherence to timelines.My contributions were vital in driving the success of high-impact clinical studies, ensuring they met the rigorous standards required for regulatory approval and market success.

As a Senior Research Associate at Guardant Health, I played a pivotal role in the development and validation of innovative NGS-based oncology diagnostic assays, focusing on target capture enrichment for rare variant and epigenetic studies. My work involved close collaboration with cross-functional teams including process engineering, clinical operations, reagent manufacturing, and bioinformatics.NGS Assay Development: Led the design, execution, analysis, and documentation of experiments to create innovative oncology diagnostic assays, enhancing assay performance through continuous assessment and optimization.Cross-functional Collaboration: Played a pivotal role in delivering assay development milestones, working closely with process engineering, operations, bioinformatics, and software teams to advance liquid biopsy sequencing technologies.Technical Expertise: Operated advanced molecular biology equipment, including automated liquid handlers and next-generation sequencing instruments, to generate high-quality data in support of new technology development.Analytical Validation: Participated in the analytical validation studies that contributed to the successful launch of TissueNext™ Tissue Genotyping v1 and v2.Mentorship and Training: Trained junior team members, fostering a collaborative and knowledgeable laboratory environment, while maintaining an organized inventory of lab supplies.Regulatory Compliance: Operated within CLIA/CAP and FDA-regulated environments, ensuring compliance with rigorous standards in a fast-paced, high-stakes setting.Effective Communication: Demonstrated a strong ability to organize, document, and present scientific data clearly and concisely to interdisciplinary teams, contributing to strategic decision-making and technical discussions.

Led gene expression analysis efforts to support basic research and development activities at Merck.• Conducted time-course studies on gene expression profiling in spleen tissue to determine effects of candida infection in mice.• Supported discovery-based efforts to understand the tumor microenvironment and acquired resistance using a resistant mouse tumor model.• Assisted in studying altered gene expression profiles in blood and tumor after treatment with specific drugs compared to control.

In my role, I was responsible for the development and launch of ClonoMap™, a novel T-lymphocyte repertoire profiling assay utilizing Next Generation Sequencing (NGS) platforms. My contributions included:Designing and validating primers to target various T-cell receptor (TCR) chains.Optimizing and validating TCR sequencing assays to enhance workflow efficiency.Utilizing bioinformatics tools such as FASTX, MIXCR, MIGEC, and VDJTOOLS for comprehensive TCR sequencing data analysis.Presenting post-analysis data and new findings to the team to guide further development.Successfully transferring the developed TCR sequencing kit to manufacturing, ensuring seamless production integration.I played a key role in developing and optimizing the ClonoMap assay for T-cell receptor repertoire sequencing on Illumina instruments, overseeing all aspects from chemistry and formulation to primer design and data analysis. The product was successfully transitioned to manufacturing, marking a significant milestone in advancing TCR sequencing technology.

At Bruker (then Core Diagnostics), I was a key team player responsible for comprehensive specimen processing and expression analysis, managing everything from sample preparation to client communication and product shipment coordination. My role involved:Performing extraction and purification of DNA, RNA, and miRNA from various tissue types, including frozen tissues, blood, and swabs, followed by nucleic acid quantification for downstream analysis.Conducting gene expression analysis from clinical trial samples using high-throughput platforms such as Fluidigm qRT-PCR and nanoString nCounter.Facilitating up to 45 pharmaceutical projects, aligning with project requirements and deadlines through effective communication with clients and internal teams.Maintaining revision control of critical documents, including QC methods, test reports, SOPs, and batch records, and providing these materials to the manufacturing team as needed.Preparing detailed reports and presentations for team review and decision-making.Developing procedures, managing multiple concurrent projects, and generating controlled documents like specifications and batch records.Utilizing expertise in nucleic acid purification, NGS, and standard molecular biology techniques (e.g., PCR, gel electrophoresis, FISH, DNA sequencing) to ensure high-quality results.Analyzing complex data sets and troubleshooting molecular biology assays, including reagent formulation and optimization.Interfacing with customers to provide updates on sample status and progress, ensuring clear and timely communication.My role required meticulous attention to detail, strong analytical skills, and a collaborative approach to manage and execute complex projects successfully.

I contributed to the culture, growth, and propagation of transgenic plants as part of the Walnut Improvement Program, focusing on developing disease-resistant rootstock. My responsibilities included:Walnut Propagation: Executed various techniques including embryo culture, micropropagation, layering, germinating open-pollinated seeds, rooting cuttings, and grafting, all performed aseptically in a highly controlled environment.Media Preparation: Manufactured a range of sterilized tissue culture media in-house to support plant growth and development.Clonal Propagation: Engaged in clonal propagation of seedlings, ensuring high-quality and uniform plant production.Tissue Culture Media Improvement: Contributed to the enhancement of tissue culture media formulations to improve plant propagation efficiency and overall plant health.My role involved working closely with advanced plant propagation techniques and contributing to the program’s goal of improving walnut rootstocks