Marcus Mahar, Ph.D. Email & Phone Number

Longmont, Co, Us

Aliso Viejo, Ca, Us

- Technical lead for RNAInsight, responsible for migration of our legacy pipeline to NextFlow/AWS, pipeline development, and product updates- Developed a python package to automate RNAinsight validation analysis, reducing BI TAT by ~4 weeks per study.- Created a CNV optimization pipeline using Snakemake and AWS to optimize CNV calling for CancerNext which improved specificity by >90%, resulting in ~$1.7M/year in direct savings.

Palo Alto, California, Us

Lead BI Scientist for exosome RNA-seq pipeline development- Languages: Python | R | Unix | SQL- BI tools: bcl2fastq | STAR | bowtie | samtools | bedtools | DESeq2 | EdgeR- Infrastructure: SGE/HPC | Snakemake | SQL

San Francisco, Ca, Us

BI Scientist responsible for ad-hoc analysis related to product development and automation of IVD and QC data analysis.- Languages: Python | R | Unix | SQL- BI Tools: fastQC | samtools | bedtools | IGV | bowtie- Infrastructure: SQL | AWS | Docker | Tableau

San Francisco, Ca, Us

ArcherDX is now InvitaeBioinformatics:-------------------- Performed and reported on biostatistical analysis in support of product development- Developed and maintained data aggregation, visualization, and reporting infrastructureLanguages: Python | R | SQL | UnixInfrastructure: AWS | Docker | LIMSGeneral:----------- Authored and reviewed protocols/reports in support of product development and V&V.

Bioinformatics:------------------ Designed and wrote analytical pipelines to compile patient sample screening data for use in verification and validation studies, research and development, and bioinformatics software improvement.Languages: R | SQLInfrastructure: AWSWet Lab:------------- Prepared hundreds of RNA, DNA, and ctDNA libraries in support of V&V studiesGeneral:----------- Conducted NGS-based experiments, performed Root Cause Analysis, and authored/reviewed protocols and reports in support of regulatory submissions to the FDA.- Worked with cross-functional teams, including R&D, Quality Control, Quality Assurance, and Software, to resolve various project-related issues.

St. Louis, Mo, Us

Thesis Lab: Dr. Valeria Cavalli, Department of Neuroscience.Focus: Utilization of RNA sequencing and phenotypic screening to identify new molecular and pharmacological targets for promoting central nervous system axon regeneration in response to traumatic brain and spinal cord injury.Bioinformatics:------------------- Developed an RNA-seq pipeline to identify high confidence molecular regulators of axon regeneration.Languages: R | UnixBI Tools: STAR | Bowtie | samtools | bedtools | subread | htseq | DESeq2 | EdgeR | MEME suiteInfrastructure: HPCWet lab:------------ Developed several medium-to-high throughput in vitro assays to enable efficient verification of molecular or pharmacological targets to promote axon regeneration- Used adeno-associated virus (AAV) for in vivo validation of novel targets in various models of traumatic nerve injury- Molecular Biology: Cloning, Library Preparation, qPCR, lentivirus production, immunofluorescence- Cell Culture: Dorsal root ganglia neurons, Cortical neurons, 293T cells, transient transfection, generation of stable cell lines, and development of high throughput phenotypic assays.- Animal Models: Mouse colony maintenance, genotyping, intrathecal injection of AAV8, sciatic nerve injury, spinal cord injury, optic nerve injury, and extensive post-operative animal care. - Microscopy: Episcopic and brightfield microscopy on both Nikon and Biotek platforms- Biochemistry: Western blottingLeadership/Communication:---------------------------------- Utilized strong scientific writing skills and breadth of scientific knowledge to secure >$80,000 in project funding. - Supervisor for one technician, five rotation students, and one undergraduate researcher.

Fargo, Nd, Us

-Teaching assistant for three courses: Foundations of Sciences (Univ. 150, Spring 2012), Introduction to Neuroscience (Psych. 260, Summer Session I), and Introduction to Neuroscience (Psych. 260, Summer Session II).

Fargo, Nd, Us

-Advisor: Dr. Mark Nawrot, Chair of Department of Psychology

Fargo, Nd, Us

-Advisor: Dr. Linda Langley

Doctor Of Philosophy - Phd, Developmental, Regenerative, And Stem Cell Biology

Bachelor Of Science - Bs, Psychology