Marina Croll Email & Phone Number

Waltham, Massachusetts, United States

Automation scientist employed by Yoh Inc and assigned at Sanofi Pasteur

Cell Line Development

Fireplex Service Lab

• Contributed to the evolution and implementation of a molecular biology workflow for generating E. coli libraries expressing candidate neoantigens to be used in ATLAS screening• Authored, edited, and assisted in reviewing SOPs and Test Methods in preparation for clinical trials• Contributed to the implementation of a fully automated workflow for library construction• Created and implemented timetables for efficient use of instrumentation and FTE hours during clinical trial library construction• Led the Synthetic Biology group’s internal freezer inventory management system to allow for improved sample chain of custody tracking• Designed and executed experiments to improve the library construction workflow• Mentored a summer intern in production of libraries for a large cohort study

Cambridge, Massachusetts

• Designed and synthesized in vitro transcribed guide RNAs to be used for large scale CRISPR screen• Performed large scale CRISPR screens in immortalized and primary cells• Optimized droplet digital PCR protocol• Provided general laboratory support, such as protocol testing and optimization, creating and maintaining lab stocks of reagents, and general cleanup and maintenance of equipment• Assisted in literature research

Andover, Ma

• Responsible for maintaining cell cultures as part of clone selection for master cell bank, and cell line suitability studies• Performed genotypic characterization for random integration and single site integration projects• Provided data for cell line suitability on genotypic level by performing DNA and RNA qualitative analysis (Southern and Northern assay), clonal sequencing, and quantitative gene copy evaluation• Provided data for cell line suitability on phenotypic level through work with AMBR, protein titer assessment, viability analysis, and viable cell density analysis• Assisted in genotypic and phenotypic data packages for BLA and LIVCA studies on biosimilars and nova proteins• Provided general laboratory support, such as protocol testing and optimization, creating and maintaining lab stocks of reagents, and general cleanup and maintenance of equipment

Waltham, Ma

• Determined the residues with which Smy1 interacts on the Bnr1-FH2 surfaceLaboratory of Dr. Bruce Goodeo Performed site-directed mutagenesis to create Bnr1 variants for testing Smy1-Bnr1 interaction o Investigated mutagenized Bnr1 for interaction with Smy1 via protein purification and in vitro assaya• Demonstrated that TALENS induce mating type switch in Saccharomyces cerevisiae Laboratory of Dr. Jim Habero Utilized nutrient selection to test activity of galactose-inducible TALENs mutants for successful cutting at a selected locuso Performed DNA ligation and Southern blot to determine if and when locus cut by TALENs is repaired• Research of Bni1 auto-inhibition Laboratory of Dr. Bruce Goodeo Created double-null Bni1 mutants to test Spa2 as an assistant to auto-inhibitiono Tested for abnormally shaped actin filaments and unusual transport in double mutants via fluorescent tagging and microscopyo Utilized in vitro assay to determine if Spa2 contributes to Bni1 auto-inhibition independently of other proteins

Columbus, Ohio

• Investigation of tRNA retrograde transport in relation to cell cycle regulation Laboratory of Dr. Anita Hoppero Prepared tagged cell-cycle proteins in mutant tRNA transport cells for protein level testingo Utilized Western blot to determine if mutation in tRNA transport caused change in cell-cycle protein levels

Master Of Science (Ms), Molecular And Cell Biology

Activities and Societies: Fencing Club (2013), Swing Dance Club (2012-2013), Laurel Moon (2012-2013)

Bachelor Of Science (Bs), Molecular Genetics

Activities and Societies: Serving with Honor - Hunger Committee (2008-2012), Analyzing the appeal of Manga (2008-2010)I conducted.

Bachelor Of Science (Bs), Mathematics