Mark Lohsen Email & Phone Number

Lexington, Massachusetts, United States

• Analyze sample VP ratios and purity using CE-SDS.
• Perform HEK host cell protein residual analysis with ELISA.

Lexington, Massachusetts, United States

• Maintain mammalian cells for AAV gene therapy knockdown potency studies.
• Determine AAV virus titers for multiple investigators using both qPCR and ddPCR.

Lexington, MA

Norwood, MA

• 1Globe Health Institute is a pioneer in the gene-silencing field with its proprietary technology platform of aiRNA.
• Manage the post-synthesis processing and de-protection of RNA oligonucleotides via solid-phase extraction.
• Assess the RNAi gene silencing efficacy via mammalian cell transfection, cellular RNA extraction, and qRT-PCR.

Boston, MA

• This lab investigates how genetic variants contribute to substance abuse, Alzheimer’s & macular degeneration.
• Genome Association: Analyzed links between genetic variants and substance abuse phenotypes. Discovered that genetic markers linked to cannabis dependence are also linked to schizophrenia.
• Programming Languages: Techniques included R programming and Python programming. Meta-analysis of multiple data sets.
• Training and Mentorship: Instructed a high school student on querying public genetic databases.
• Awarded Transformative Training Program in Addiction Sciences (TTPAS) grant.

Boston, MA

• This lab investigates bacterial outer membrane proteins and their applicability to vaccine enhancement.
• Colony Management: Bred, genotyped, and ultimately engineered conditional knockout mice in all 3 antigen-presenting cell types.
• Immunization Comparison: Immunized mice with a wide variety of vaccine adjuvants. Through sub-mandibular bleeds and ELISA, discovered that Neisserial porin PorB is as effective as current commercial adjuvants.
• Training and Mentorship: Supervised one graduate student and three undergraduate students on a variety of techniques including SDS-PAGE, ELISA, and protein purification.

Frederick, MD

• This lab studies the mechanisms behind blindness and deafness in animals.
• Amplified, sequenced and analyzed Felis catus DNA in search of indicative mutations for retinal atrophy and congenital deafness.
• Techniques included PCR and gene sequence analysis.

Hershey, Pennsylvania, United States

• This lab studies the interactions between viruses and host cells using retroviruses as model systems.
• Observed avian cells transfected with mutated RSV plasmid under the confocal microscope. Correlated experiments between the confocal and transmission electron microscope.
• Techniques included bacterial cloning, viral transfection, confocal microscopy and electron microscopy.

• This lab investigates the mechanisms of widespread viruses with the goal of discovering viral inhibitors.
• Performed genetic recombination experiments on cultured HEK cells to investigate genetic complementation of adenovirus genes.
• Techniques included qPCR, PCR, agarose gel electrophoresis and virus isolation.

Master Of Arts (M.A.), Biochemistry

Bachelor Of Science (B.S.), Biochemistry And Molecular Biology

Activities and Societies: Alpha Phi Omega