Mark Lohsen Email & Phone Number

Lexington, Massachusetts, United States

• Analyze sample VP ratios and purity using CE-SDS. • Perform HEK host cell protein residual analysis with ELISA.

Lexington, Massachusetts, United States

• Maintain mammalian cells for AAV gene therapy knockdown potency studies. • Determine AAV virus titers for multiple investigators using both qPCR and ddPCR.

Lexington, Ma

Norwood, Ma

1Globe Health Institute is a pioneer in the gene-silencing field with its proprietary technology platform of aiRNA. • Manage the post-synthesis processing and de-protection of RNA oligonucleotides via solid-phase extraction. • Assess the RNAi gene silencing efficacy via mammalian cell transfection, cellular RNA extraction, and qRT-PCR.

Boston, Ma

This lab investigates how genetic variants contribute to substance abuse, Alzheimer’s & macular degeneration. • Genome Association: Analyzed links between genetic variants and substance abuse phenotypes. Discovered that genetic markers linked to cannabis dependence are also linked to schizophrenia.• Programming Languages: Techniques included R programming and Python programming. Meta-analysis of multiple data sets. • Training and Mentorship: Instructed a high school student on querying public genetic databases. • Awarded Transformative Training Program in Addiction Sciences (TTPAS) grant.

Boston, Ma

This lab investigates bacterial outer membrane proteins and their applicability to vaccine enhancement. • Colony Management: Bred, genotyped, and ultimately engineered conditional knockout mice in all 3 antigen-presenting cell types. • Immunization Comparison: Immunized mice with a wide variety of vaccine adjuvants. Through sub-mandibular bleeds and ELISA, discovered that Neisserial porin PorB is as effective as current commercial adjuvants. • Training and Mentorship: Supervised one graduate student and three undergraduate students on a variety of techniques including SDS-PAGE, ELISA, and protein purification.

Frederick, Md

This lab studies the mechanisms behind blindness and deafness in animals.• Amplified, sequenced and analyzed Felis catus DNA in search of indicative mutations for retinal atrophy and congenital deafness. • Techniques included PCR and gene sequence analysis.

Hershey, Pennsylvania, United States

This lab studies the interactions between viruses and host cells using retroviruses as model systems. • Observed avian cells transfected with mutated RSV plasmid under the confocal microscope. Correlated experiments between the confocal and transmission electron microscope. • Techniques included bacterial cloning, viral transfection, confocal microscopy and electron microscopy.

This lab investigates the mechanisms of widespread viruses with the goal of discovering viral inhibitors. • Performed genetic recombination experiments on cultured HEK cells to investigate genetic complementation of adenovirus genes. • Techniques included qPCR, PCR, agarose gel electrophoresis and virus isolation.

Master Of Arts (M.A.), Biochemistry

Bachelor Of Science (B.S.), Biochemistry And Molecular Biology

Activities and Societies: Alpha Phi Omega