Work with staff scientists and clinicians in translational hematologic research, specifically with Fanconi anemia viral gene therapy in vitro and in vivo murine modelDeplete hematopoietic stem cells with targeted conjugated-antibody toxin and assess engraftment of gene modified infusion product in bone marrow to correct aberrant Fanconi genes using flow cytometry and colony forming assays as readoutsInvolved in production and quality testing of research-grade and clinical-grade Lentiviral Vectors (LV) and Adeno-Associated Vectors (AAV) to ensure high-quality manufactured productsPerform transfection in cultured HEK293T cells for vector production and determine titer using transduction in HT1080 cells with viral vectors in Vector Core labIdentify viral vector integration site of transgene product in genome via next generation sequencing

Support senior scientists to design and run hands-on experiments for sample lysis, nucleic acid extraction, amplification, and identification of infectious microbiological samples from mammalian bodily fluidsResearch and carryout carefully designed experiments for cell culturing clinical bacterial strains (Gram-postitive and Gram-negative; BSL I & II) and storing cultures as glycerol stocksPerform nucleic acid amplification using standard PCR, RT-qPCR and drop digital PCR measurements and multiplexed reactionsExecute biotinylation of antibody fragment to streptavidin paramagentic beadsContribute to weekly Molecular Biology R&D data-driven meetings and monthly project updates presented to fellow lab members and executive teamResearch primary literature to understand concepts and methods in the fields of immunology, microbiology, and molecular biology for product developmentWrite standard operating procedures (SOP) and protocols to undertake nucleic acid extractions from clinical samplesInvolved in performing reagent quality control, generating and testing of designed experiment controls and standardsParticipate in assay product development related activitiesRecord, summarize, and interpret experiment data

Follow and update SOPs to perform molecular cloning with E. coli vector using electroporation and heat-transformation methodsDevise methods to remove background colonies that contain background template with restriction enzymes and test clone vector transformation using GFPCollaborate with other associates and scientists in process development team to transfer methods for manufacturing production scale-upPrimer and construct design using DNA analysis software (SnapGene, Geneious, MacVector)Plasmid Miniprep and DNA purification using column and bead purification kitsQC analysis of purified DNA using Agilent 2100 Bioanalyzer and quantitation using Qubit Assay KitSample library preparation for Next Generation Sequencing (Illumina HiSeq and MiSeq)Exposed to method editing and deck layout design for Hamilton liquid handling system to perform automation related to molecular cloning

Taught buffer-exchange dialysis, ion-exchange and hydrophobic chromatography, FPLC (AKTA Pure 25; UNICORN control system), protein assays (SDS PAGE, BCA, ELISA), tangential flow filtration, and LC-MS/MS sample prep techniques to senior engineering students in a collaborative student project with Genentech to characterize a Lucentis-like F(ab’)2 antibody fragmentAssisted professors with equipment, reagent, and buffer preparations and synthesis of data resultsReviewed biochemical and molecular concepts in classroom and in the laboratory weekly with students

•Oversaw operations of station DJs and maintain excellent quality of programs on daily basis•Effectively organized station events such as concerts and community outreach effortsWorked within budget to: communicate with campus admin, book musicians and work to settle contracts/riders, hire event staff and security, and promote event on and off campus•Collaborated with station manager and other directors in meeting expectations set early in the year such as increase station presence, implement new technology, track progress of DJs, and become the leading underground music student-run station in Nevada

•Maintain social media sites and newsletter to create relationships with customers via online marketing campaigns.•Plan, organize, and coordinate in-store events that encourage customer involvement in an effort to shift outdated store perceptions.•Manage the day-to-day marketing tasks of the university bookstore such as oversee marketing assistants and internship program.•Facilitate and interpret market research of store and industry.

•Increase membership through strategic year-round recruitment methods.•Develop fraternity brand grounded on founding principles to maximize community and General Headquarters recognition.•Brand and create recruitment events to entice potential members.•Maintain budget for recruitment events.•Work with the executive board to focus on and execute the goals of the fraternity on campus.

•Coordinated concerts at the university by way of booking musicians and gauging student interests. Looked at demographic data and booked artists accordingly.•Effectively managed budgets dealing with artist contracts and event expenses. Staffed personnel for event including production and security teams.•Worked with professionals in the music industry on the local and national level. Maintained strong relationships with university and local community.