Mark Garrett Email & Phone Number

Overland Park, Ks, Us

De Soto, Kansas

Desoto, Ks

* Part of a team responsible for support of existing products at the site’s production department and transfer of new products from the site’s R&D department. * Planned and executed process improvements for several existing bacterial products. * Lead R&D to production product transfer for bacterial and viral vaccines, including R&D feasibility studies, process development, financial evaluations, specification and bidding of custom and COTS equipment, writing of SOPs for production, product transfer studies. * Lead the design and commissioning of a PD/PI cell culture/virology laboratory, a set of 10L benchtop fermenters, and a 100L fermenter. Participated in the design and commissioning of a 50L SUB. * Planned and executed a wide range of media development, growth optimization, inactivation, purification, and vaccine blending studies* Served as a technical resource in all phases of development for R&D.

De Soto, Ks

* Participated in all aspects of product development and USDA/CVB licensure including strain selection; process development, process optimization and transfer; bulk and final product potency assays (in vitro and small animal assays); challenge model development; surrogate animal studies, animal efficacy, duration of immunity and field safety studies; development of qPCR, bacteriological, virological and immunological assays to evaluate vaccine efficacy. * Assisted in a variety of product improvement activities for existing swine, cattle, and equine US vaccine lines. Re-developed existing ELISAs to meet new USDA requirements. Replenished/qualified assay reagents. Developed and executed alternative assays to monitor vaccine stability using a variety of assay techniques, including SDS-PAGE/Western blot and HPLC. * As head of R&D Quality Council, responsible for devising/implementing SOP system, writing/reviewing SOPs, improving calibration & maintenance programs for department. * Trained researchers in use of a wide range of laboratory and pilot-scale methods and equipment.

Dallas, Tx

• Performed cytotoxicity and virus-inhibition cell-based assays in support of a novel technology to design small molecule drugs. Increased throughput while decreasing assay variability.• Assisted in development and routine execution of a FRET-based enzyme inhibition assay, including transfer of the assay to a Biomek 2000 liquid handler.• Performed routine data analysis and reporting. Also contributed to the programming of a data analysis platform for assay data (involving R, PERL, SoftMax Pro, EnzFitter, and MS Access).• Assisted in-house crystallography team in purification of recombinant proteins.• Implemented toxicity, bioconversion, stability assays for a series of novel antibiotics.• Consolidated equipment and lab space, executed waste disposal, supervision of other employees, and safety inspections during company-wide downsizing.

Dallas, Tx

• Responsible for growth and PCR screening of bacterial libraries of mouse chromosomes. Operated Shimadzu mini-prep robot.

Lewisville, Tx

• As intern, received orders, operated oligonucleotide synthesizers, purified and prepared oligonucleotides for shipping.

Ma, Microbiology

• Coursework included bacterial pathogenesis, virology, immunology, molecular biology, biochemistry, and biometry. • Gained experience.

Ba, Biology

Activities and Societies: Habitat for Humanity • Coursework included: microbiology, bacteriology, medical bacteriology, cell biology.