Martina Chambers Email & Phone Number

Vancouver, British Columbia, Canada

Vancouver, British Columbia, Canada

Designs and performs a variety of specialized laboratory procedures, including particle conjugation, particle characterization, and cell separation techniques. The major focus of my position is on the analytical characterization of particles manufactured for various cell separation kits. Also function as the particle chemistry lab manager.Key Responsibilities:• Conjugation of polymers to a particle surface using established bioconjugation reactions.• Characterization of polymer-coated particles using zeta potential, dynamic light scattering (DLS), total organic content (TOC), CPS disc centrifuge particle sizer, microscopy, FTIR and thermogravimetric analysis (TGA). Advanced analytical techniques to study particle surfaces include field flow fractionation (FFF) and quartz crystal microbalance with dissipation monitoring (QCM-D). Also skilled in performing various absorbance and fluorescence based assays to quantify functional groups on particle surfaces.• Analysis of particle performance in cell separation applications, including flow cytometry, cell counting, and handing of biohazardous material using aseptic techniques.• Project management experience including day-to-day experiment planning for myself and co-op students, literature searches and long-term project planning (>6 month timeline).• Responsible for management, maintenance, and training on key laboratory instrumentation, including a Q500 TGA, TOC-L TOC Analyzer, CPS disc centrifuge Particle Sizer, LUMiSizer Particle Sizer, Brookhaven Particle Size Analyzer, FFF, QCM-D and both top loading and analytical balances.• Responsible for the day-to-day management of the particle chemistry lab, including inventory management, instrument maintenance and organization of the laboratory following the 5S methodology.

Vancouver, British Columbia, Canada

Designs and performs a variety of specialized laboratory procedures, including particle conjugation, particle characterization, and cell separation techniques. The major focus of my position is on the analytical characterization of particles manufactured for various cell separation kits. Also function as the particle chemistry lab manager.Key Responsibilities:• Conjugation of polymers to a particle surface using established bioconjugation reactions.• Characterization of polymer-coated particles using zeta potential, dynamic light scattering (DLS), total organic content (TOC), CPS disc centrifuge particle sizer, microscopy, FTIR and thermogravimetric analysis (TGA). Advanced analytical techniques to study particle surfaces include field flow fractionation (FFF) and quartz crystal microbalance with dissipation monitoring (QCM-D). Also skilled in performing various absorbance and fluorescence based assays to quantify functional groups on particle surfaces.• Analysis of particle performance in cell separation applications, including flow cytometry, cell counting, and handing of biohazardous material using aseptic techniques.• Project management experience including day-to-day experiment planning for myself and co-op students, literature searches and long-term project planning (>6 month timeline).• Responsible for management, maintenance, and training on key laboratory instrumentation, including a Q500 TGA, TOC-L TOC Analyzer, CPS disc centrifuge Particle Sizer, LUMiSizer Particle Sizer, Brookhaven Particle Size Analyzer, FFF, QCM-D and both top loading and analytical balances.• Responsible for the day-to-day management of the particle chemistry lab, including inventory management, instrument maintenance and organization of the laboratory following the 5S methodology.

Vancouver, Canada Area

Designs and performs a variety of specialized laboratory procedures, including particle conjugation, particle characterization, and cell separation techniques. The major focus of my position is on the analytical characterization of particles manufactured for various cell separation kits. Also function as the particle chemistry lab manager.Key Responsibilities:• Conjugation of polymers to a particle surface using established bioconjugation reactions.• Characterization of polymer-coated particles using zeta potential, dynamic light scattering (DLS), total organic content (TOC), CPS disc centrifuge particle sizer, microscopy, FTIR and thermogravimetric analysis (TGA). Advanced analytical techniques to study particle surfaces include field flow fractionation (FFF) and quartz crystal microbalance with dissipation monitoring (QCM-D). Also skilled in performing various absorbance and fluorescence based assays to quantify functional groups on particle surfaces.• Analysis of particle performance in cell separation applications, including flow cytometry, cell counting, and handing of biohazardous material using aseptic techniques.• Project management experience including day-to-day experiment planning for myself and co-op students, literature searches and long-term project planning (>6 month timeline).• Responsible for management, maintenance, and training on key laboratory instrumentation, including a Q500 TGA, TOC-L TOC Analyzer, CPS disc centrifuge Particle Sizer, LUMiSizer Particle Sizer, Brookhaven Particle Size Analyzer, FFF, QCM-D and both top loading and analytical balances.• Responsible for the day-to-day management of the particle chemistry lab, including inventory management, instrument maintenance and organization of the laboratory following the 5S methodology.

Vancouver, Bc

Supervisor: Prof. Stephen G. WithersResponsible for the synthesis, full characterization, and analysis of both carbohydrate and glycolipid polymers, with a focus on cellulose. The polymers were prepared using a combination of organic synthesis and enzymatic synthesis. Major Projects:• Synthesis of soluble cellulose oligomers and cellulosic glycolipids containing 6-8 glucose units, with the use of engineered enzymes.• Synthesis and characterization of isotopically labeled cellulose containing 4-6 glucose units.Techniques and Skills Routinely Utilized:• Synthesis and purification of carbohydrate polymers and glycolipids using a variety of organic synthesis techniques, including reflux, acid/base workups, flash column chromatography, reverse phase chromatography, and thin layer chromatography (TLC).• Characterization of synthesized products using nuclear magnetic resonance (NMR – 1D and 2D), electrospray ionization mass spectrometry (ESI-MS), and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). • Perform the expression of proteins and enzymes in E. coli cells. Subsequently, purified and characterized proteins and enzymes using affinity chromatography, bicinchoninic acid (BCA) assay, UV-VIS spectroscopy, MALDI-MS, and polyacrylamide gel electrophoresis (PAGE). • Prepared weekly reports on research progress, as well as performed data processing and interpretation of results using system software and office suites.

Vancouver, Bc

Responsible for supervising undergraduate students in a laboratory period. Also, responsible for marking laboratory reports, midterm exams, and final exams. Courses: CHEM 111 (Principles of Chemistry), CHEM 113 (Principles of Chemistry II), CHEM 121 (General Chemistry - Structural Chemistry), and CHEM 123 (General Chemistry - Physical and Organic Chemistry)

Berkeley, Ca

Supervisor: Dr. Frantisek SvecPreparation and characterization of novel monolithic TLC plates for the fast and efficient separation of complex protein mixtures according to their isoelectric points. • Preparation, testing, maintenance and use of polymerization solutions for laboratory. • Preparation and study of polymeric monolith TLC plates using photochemical radical initiation. • Surface modification of prepared polymer surfaces and structures using ultraviolet (UV) photografting.• Characterization of monolithic TLC plates using a contact angle goniometer, x-ray photoelectron spectroscopy (XPS), and MALDI-MS.• Responsible for the training and supervision of an undergraduate summer student. Also, maintained, organized instrument bookings, and held training sessions on a contact angle goniometer instrument.• Performed data processing and interpretation of results using system software and office suites.

Berkeley, Ca

Intel’s Molecules for Advanced Patterning (MAP) ProgramSupervisors: Dr. James Blackwell and Dr. Frantisek SvecAnalysis of photoacid generators and polymers for the preparation of novel micro-transistors and consumer electronics. • Prepared polymer samples for silicon wafer coating and exposures at the Advanced Light Source (a US national synchrotron). • Used a variety of standard and advanced analytical methods to purify and analyze polymers and photoacid generators, including NMR, gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC, reversed phase and size exclusion), MALDI-MS, UV-VIS spectroscopy, and dektak surface profiling. • Handled sensitive polymer samples using a UV reactor, schlenk lines, or a glove box. Also, prepared and stocked synthetic and analytical solutions for a synthesis lab.• Employed data processing and interpretation of results using system software and office suites.

Edmonton, Ab

Supervisor: Prof. Charles LucyHonours Thesis Title: Studies of Mixed Surfactant Bilayer Coatings for Capillary Electrophoretic Separations of Proteins • Used, prepared, and characterized novel charged surfactant coatings for separations of protein mixtures using capillary electrophoresis.• Personally operated and maintained commercial and modified CE systems (>200 instrument hours logged).• Prepared and utilized complex protein mixtures, buffers, and mixed surfactant solutions (focus on phospholipids and charged organic surfactants).• Analysis of mixed surfactant vesicles using dynamic light scattering.

Edmonton, Ab

Supervisor: Prof. Wolfgang JaegerNSERC Undergraduate Student Research Award (USRA) recipient.Performed spectroscopic and computational studies of the weakly bound He-FCN dimer and weakly bound He-H2-OCS trimer using microwave spectroscopy.• Operated an in-house, state-of-the-art Fourier transform microwave spectrometer independently and supervised (>200 instrument hours logged).• Prepared gas samples using advanced sample introduction methods, including arc discharge and electrodeless discharge.• Performed advanced kinetic and thermodynamic chemical calcuations using Molden, MOLPRO, and Jacobi.

Edmonton, Ab

Supervisor: Prof. Glen LoppnowStudy of mechanisms that can be used to repair damaged, cross-linked DNA polymers.• Designed DNA strands used for cross-linking studies using Oligo Analyzer software.• Prepared complex solutions using biologically relevant conditions.• Measured DNA absorbance properties using a UV-VIS spectrometer coupled to a temperature-controlling device.

Master Of Science (M.Sc.), Organic Chemistry

Activities and Societies: Chemistry Graduate Student Society Secretary (2011-2013) Master's Thesis supervisor: Prof. Stephen G. Withers.

Bachelor Of Science With Honours (Bsc Honours), Chemistry

Activities and Societies: Undergraduate Chemistry Society Member (2007-2010), VP Admin of Undergraduate Chemistry Society (2009-2010) My.