Matt Heidtman, Phd Email & Phone Number

Saint Paul, MN, US

Minneapolis, Minnesota, US

• Summary: At Bio-Techne, I am an R & D manager/senior scientist working in the antibody development and innovation group. I am leveraging molecular biology and cell line engineering technology to develop new products.
• Leads mammalian cell line development campaigns for commercial-scale production of recombinant antibodies. Engineers cells for robust, stable antibody production using cutting edge genome modification technologies.
• Designs, engineers and constructs expression vectors for efficient production of target molecules (both stable and transient expression platforms)
• Conceives screening strategies to identify high-producing cell lines
• Engineers antibodies for specific applications, including humanization, isotype swapping, affinity tagging and Fc region engineering
• Manages small teams of research associates to accomplish project goals.

Minneapolis, Minnesota, US

Minneapolis, Minnesota, US

• Summary: Successfully engineered bacteria, fungi, and mammalian cells for the efficient production of bioactive human milk oligosaccharides (HMOs) utilizing pathway engineering, molecular biology and genetic.
• Engineered microbial hosts (bacteria, fungi) for commercial-scale production of HMOs via fermentation using both plasmid-based and genome engineering strategies
• Engineered CHO cells and fungi to stably produce specific cell-surface glycoproteins
• Identified, cloned, and characterized novel, proprietary glycosyltransferases for production of HMOs in microbial hosts
• Demonstrated robust large-scale production of oligosaccharide products (g/L titers) via bioreactor experiments in close collaboration with fermentation engineer (2L and 10L bioreactors)
• Successfully developed and implemented in vitro cell-based assays to assess HMO targets/function (bacterial/host-cell binding assays)

Medford, Massachusetts, US

• Summary: Investigated molecular mechanisms underlying the ability of the human bacterial pathogen Legionella pneumophila to subvert host cell innate immunity. Discovered a novel group of L. pneumophila virulence.
• Identified novel secreted virulence proteins produced by Legionella pneumophila using a large scale yeast expression strategy (over 100 heterologous proteins successfully expressed)
• Expressed heterologous proteins in mammalian cell lines by transient transfection; analyzed localization by immunofluorescence microscopy and subcellular fractionation
• Developed and utilized biochemical assays to identify binding partners/targets of virulence proteins
• Used cell-based assays and infection models to show virulence proteins manipulate host cell vesicle trafficking/signaling pathways by distinct molecular mechanisms
• Supervised/directed research projects of one summer student and three graduate students; trained and mentored technical staff

Hanover, NH, US

• Summary: Investigated molecular mechanisms underlying vesicle traffic/protein transport between the endoplasmic reticulum (ER) and the Golgi complex. Discovered an essential role for the highly conserved integral.
• Developed and implemented cell-based assays to show a critical role for Yip1p in transport vesicle formation from the ER
• Developed biochemical assays showing that Yip1p forms a complex with a second highly conserved integral membrane protein termed Yos1p
• Provided evidence that Yos1p is efficiently incorporated into ER-derived transport vesicles
• Developed assays for the analysis of Yip1p function in early secretory traffic; optimized reconstituted transport assays to measure ER-to-Golgi transport in vitro
• Successfully identified amino acids critical for the function of the Yos1p in ER-to-Golgi transport using targeted mutagenesis

New Haven, CT, US

• Investigated subcellular localization of the yeast (S. cerevisiae) proteome using transposon mutagenesis and immunofluorescence microscopy

Boston, MA, US

• Investigated molecular mechanisms/signalling pathways underlying lung inflammation.

Ph.D., Molecular And Cellular Biology

B.A., Biology