Matthias Rieb Email & Phone Number

Dreieich, Hesse, Germany

Clinical manufacturing plantTasks:-Change control-Qualification/Validation-General compliance

Combined Project in AG Pohl: Enzymes and Biosensors & AG Kohlheyer: Microscale BioengineeringDevelopment of applications for FRET based biosensors in microfluidic chip systems.The aim of this project is to develop FRET-based biosensors for application in single cell analysis. Thereby a quantitative insight into substrate uptake and production of amino acids shall be achieved.Methods: -Surface modification of microparticles-Production and purification of biosensors-Genetic engineering-Production of microfluidic chips-Singele cell cultivation-Fluorescence lifetime imaging-Fluorescence microscopy-Lifecell imaging-Spectroscopy

Jülich

AG Rother Biocatalysis GroupTask: Development and establishment of a fermentation process for highly active transaminasesTechniques: -DASGIP fermentation-Visual basic (fermentation script writing)-Fermentation optimization

Jülich

Prepared at AG Rother Biocatalysis GroupTitle: Technology transfer and optimization of an industrial, biocatalysis based (R)-1-phenylethylamine synthesisTechniques:-Technology transfer-Process optimization-Development of process analytics-Analysis of aromatic compounds via HPLC and GC-Analysis of imines and volatile compounds via NMR and GC-Fermentation-Micro aqueous reaction system-Building lab scale reactors-Biocatalysis-Distillation-Extraction

Kreisfreie Stadt Aachen Und Umgebung, Deutschland

AVT - Chair of Process Fluid EngineeringTask: Assisting in a project of the BioSC Bioeconomy Science Center to purify biosurfactants at the Chair of Process Fluid Engineering.Techniques: -Production of the biosurfactants in liter scale-Process development-Primary recovery of fermentation broth-Selective separation of proteins via precipitation and extraction-Foam fractionation-Purification of fusion proteins via affinity chromatography-Analysis of biosurfactants via UHPLC and Corona detector-Analysis of glycolipids via TLC-Protein analysis via SDS PAGE and spectrometry

Kreisfreie Stadt Aachen Und Umgebung, Deutschland

AVT - Chair of Process Fluid EngineeringTitle: Commissioning of a chromatography device and determination of adsorption isotherms of chosen hydrophobic adsorbentsTechniques:-Commissioning of a chromatography device (Äkta Avant 25)-Determination of adsorption isotherms with dynamic and static methods-Writing of chromatography methods-Writing of standard operating procedures

Mainz Und Umgebung, Deutschland

III. Medizinische Klinik und PoliklinikAG KindlerTask: Modifying cell lines to asses the effects of personalized medication in leukemia patientsTechniques: -Cell culture (single cell cloning of adherent and suspension cells) -CRISPR/CAS9 (homologous recombination, knockouts) -Design of primers, sgRNA's and ssODN's -FACS (cell cycle and live cell analysis) -In-vitro transcription -MTT-assay -RNA isolation -Semi-quantitative RT-PCR -Western blot

Mainz Und Umgebung, Deutschland

Prepared at the AG RoukosTitle: Generation and validation of cellular CRISPR/Cas9 systems to study the biogenesis of therapy-related oncogenic MLL translocationsTechniques: -DNA isolation (plasmid, genomic DNA) -Cell culture (human cells) -Cloning (restriction, ligation, recombination) -Confocal microscopy (Immunofluorescence) -CRISPR/Cas9 -High-Content screening microscopy (Opera Phenix High-Content Screening System) -Site directed mutagenesis -Transformation, transfection (lipofection, electroporation), transduction (lentivirus) -Western blot

Birkenfeld

Task: Typing of leukemia patients and stem cell donors:Techniques:-DNA Isolation from saliva and whole blood samples -PCR-Sanger sequencing-Bioinformatic analysis of sequencing results-quality assurance2 advanced trainings in HLA-Workshops

Doctor Of Philosophy - Phd, Chemistry

Master Of Science - Ms, Biotechnology, 1.1

Area of specialisation: process engineering coupled with white biotechnology Additional qualifications: 16 extra credit points

Bachelor Of Science - Bs, Biotechnology, 1.6

Activities and Societies: Math Tutor for preparatory courses in 2014 and 2015Lab work in microbiology, cell biology, biochemistry.