Involved in transient expression of Biotherapeutical proteins in HEK and ExpiCHO system, Pseudovirus generation and cell based assays.

Handling multiple client relationships, generating stable cell lines, bio-therapeutic protein production.

Vaccine development and production

Cloning of viral vectors to generate AAV vector as Gene Therapy product. Generating AAV vectors in adherent and suspension cell lines using triple transfection.Established serum free culture for generation of AAV to increase virus productivit

Gained upstream expertise in animal cell culture for the production of biotheapeutics and monoclonal antibodies from small to large scale transfections.Developed monoclonal cell lines using lentiviral and non-lentiviral approaches.Deployed hybridoma technology and serum weaning to generate hybridoma monoclones. Experience in FACS, cell sorting, clone pix, octet, immunostaining.

Gained upstream expertise in animal cell culture for the production of biotheapeutics and monoclonal antibodies from small to large scale transfections.Developed monoclonal cell lines using lentiviral and non-lentiviral approaches.Deployed hybridoma technology and serum weaning to generate hybridoma monoclones. Experience in FACS, cell sorting, clone pix, octet, immunostaining.

Studied Th17 and Treg paradigm in auto-immune colitis using mouse C57/BALB/C mouse model systemsEstablishment of CCR9 and CCL25 over expressing cell linesAssessed transcriptional and molecular regulation of Th17 gene locus in Th17cells during autoimmunity and inflammationExperience in Animal handling, cloning, animal cell culture. Bio-informatics. Experience in FACS and sorting, western blotting, immunostaning, sectioning and mounting tissue and confocal microscopy.