Mariana Castillo Email & Phone Number

Durham, North Carolina, United States

Managed cross-functional process development projects to improve core technologies at AltisDrafted Quality Control metrics for human intestinal primary cellsPerformed all tasks included under Research Associate description

Durham, North Carolina, United States

Planned and executed QC assays and analyzed data to ensure quality standards were metAuthored Certificates of Analysis for Altis generated cell lots that passed QCServed as Interim Cell Operations and QC Manager in charge of generating all products manufactured by AltisServed as Interim QC Manager to ensure all Altis manufactured products met established QC metricsServed as lab trainer for new employees for cell culture and lab-based techniquesOptimized and established protocols to establish consistency across Altis productsWorked cross-functionally with Commercial team, research and development, and Business development to provide expertise in culturing primary cellsPerformed all tasks included under the Research Technician description

Durham, North Carolina, United States

Established Human intestinal stem cell lots from various donors and all regions of the human intestine. Conducted Quality Control assays, such as TEER measurements, permeability assays, and immunostaining. Voluntarily worked on the weekend for six months to maintain the company’s weekend culture work when the company lacked the staff. Maintained department inventory and ordered products when necessary. Maintained a detailed recollection for all procedures and experiments conducted. Prepared various buffers, media , and reagents for departmental use

Raleigh, North Carolina, United States

Planned and executed an independent project of adapting C710 and C636 mosquito cells to grow in serum free media by gradually increasing the percentage of serum free media. Assessed the cells’ health and progress on a daily basis and used critical thinking to determine when to increase the percentage of serum-free media. Maintained a detailed lab notebook recording observations and progress made each day as well as questions and hypotheses for potential further research.

Raleigh, North Carolina

Routinely passaged mammalian cells and human monocyte cells. Regularly autoclaved equipment as well as trash for proper disposal. Prepared sterile media and solutions for laboratory use. Conducted multiple plaque assays using baby hamster kidney cells and Sindbis virus and calculated the titer of the virus samples using the plaque assay results.

Raleigh, Nc

Assisted NCSU Organic Chemistry Students in achieving their target grade in the class by guiding the students to gain the essential skills to excel in the course independently.