Lead a team of 4 to 10 members for upstream and downstream viral vector processing.- Completed on time internal tech transfer and external client deliverables.- Facilitated scientific collaborations with vendors to test new equipment and materials.- Established client readiness through hiring, document generation, and equipment acquisition as part of site start up activities.- Developed associates by guiding them through development of a suspension AAV process, resulting in improved titers.- Planned successful events for global and local DEI&B council.

PD upstream engineer for commercial and small-scale processes during start-up of a gene therapy facility for AAV adherent process.- Demonstrated an equivalent scale down model that allowed for further process characterization.- Designed studies and collaboratively lead a team of three to execute studies, then analyzed and presented data and findings.- Collaborated across sites and functions to decrease impurities during the process leading to higher quality product.- Executed analytical assays (ddPCR, qPCR, ELISA) to support projects and trained others on ddPCR leading to shorter sample turnaround time.- Participated in the Core Green Team focusing on sustainability and environmental efforts.

Lead researcher in the Downey lab exploring the role of PTPalpha in pulmonary fibrosis.- Collaborated with outside institutions and industry on pre-clinical testing of inhibitors for off label treatment of fibrosis, leading to next phase funding and testing.- Optimized protocols for fluorescent Western blot imaging, including calculations sheet, protein transfer, membrane type, and antibody selection.- Established RT-PCR of mRNA methods and tracking system for ensure primers for housekeeping genes remained consistent across all stimuli and experiments.- Designed studies utilizing siRNA knockdown in primary human lung fibroblast cells to compare changes in response to stimuli.- Maintained equipment and was responsible for all materials ordering, requiring an understand of other researchers needs and rate of use.

Discovery function within the R&D group screening for monoclonal antibody and vaccine applications in canine and poultry.- Developed a PCR assay detecting the presence of a recombinant virus vaccine in poultry that was approved by the USDA for study evaluation and monitoring.- Performed cloning and validation of plasmids for transfection to produce recombinant antibodies for use in canine obesity studies.- Executed titration ELISA of sera supporting atopic dermatitis in canines.- Assisted with animal studies primarily using mouse models, or poultry target species, with some exposure to canine studies.- Supported the site by participating in IACUC Post Approval Monitoring committee and the Rees monitoring system for equipment.

Primary researcher focused on contract testing and exploring how and what causes Staphylococcus aureus to gain and loose antibiotic resistance.- Conducted phylogenetic strain type testing for hospitals and industry by PFGE, sequencing, and MSLT of MRSA, Clostridium difficile, Pseudomonas aeruginosa, and Escherichia coli.- Established and maintained a sample intake and organization system, standardizing sample naming and preventing labeling errors during testing.- Assessed and compared the Agilent 2100 Bioanalyzer instrument for strain typing of C. difficile against PFGE analysis.- Evaluated River Diagnostics SpectraCell RA compared to the gold standard of PFGE for MRSA strain typing and presented findings at an international conference.- Banked bacterial isolates submitted by large pharmaceutical company as a backup storage location and would retrieve and grow banked samples when requested.- Prepared shipments of plates and swabs for client collection of samples.- Trained and supervised daily activities of undergraduate students, increasing their responsibilities as skills and knowledge were obtained.- Maintained all equipment and ordered materials.

Conducted research on early events of HIV-1 cellular infection while maintaining a full graduate course load most of the time. - Purified reverse transcription complexes (RTC) and preintegration complexes (PIC) using velocity sucrose gradient centrifugation - Produced HIV-1 pseudotyped virus by transient transfection followed by purification and concentration with centrifuge filters. - Extracted viral DNA and RNA for detection by qPCR and RT-PCR respectively, leading to further exploration of locations of RTC and PIC in gradient fractions.- Developed and optimized an in vitro integration assay detected with nested PCR/qPCR to confirm purified preintegration complexes were active.- Confirmed reverse transcription complexes were active by optimizing an enzymatic incorporation assay utilizing 32P.

- College Program recreation cast member- Licensed deep water lifeguard- Fireworks cruise boat driver- Majority of time spent between The Contemporary, Fort Wilderness, The Wilderness Lodge, The Grand Floridian, and The Polynesian resorts. - Duties included ensuring the safety of Guests in and around the pools, monitoring pool slides, assisting Guests with boat rentals, organizing children's activities, and cleaning, setting up, and captaining Bay Lake fireworks cruises.

- Summer research in material science and engineering- Focused on increases strength of borate glasses for use as a bone substitute- Batched and quenched glasses