Graduate Research Assistant
Evaluated the genetic relationship and the host ranges of the downy mildews of hop (Pseudoperonospora humuli) and cucurbit (P. cubensis).Established and maintained detailed and accurate records in laboratory notebook.Maintained computer files of detailed and accurate research findings.Maintained isolates of the obligate parasites P. humuli and P. cubensis via weekly transfers.Performed and evaluated cross-inoculation studies.Alerted supervisors to procedural, technical, and logistical problems.Prepared complex scientific reports, manuscripts, and scientific presentations.Communicated and collaborated with technical and scientific staff as part of a multi-disciplinary team.Performed DNA extractions from various types of organismal tissue.Performed polymerase chain reaction (PCR).Programmed, operated, and maintained thermal cyclers used for PCR.Contributed to the ongoing development of quality assurance and quality control guidelines and standards for the analysis of PCR reactions to ensure accuracy and consistency in genetic studies.Used sequence analysis software to interpret sequencing chromatograms.Used sequence analysis software to analyze DNA sequences against in-house and GenBank databases.Performed general molecular biological techniques including bacterial plasmid isolation, DNA quantification, and basic DNA cloning.Operated various types of research equipment such as incubators, microscopes, sterile hoods, chemical hoods, autoclaves, and spectrophotometers.Assembled, evaluated, and prepared field and laboratory data for tabulation and analysis.Followed laboratory and research protocols in collecting, measuring, and reporting scientific data.Prepared sequencing data for sequence alignment and statistical analysis.Prepared DNA samples for sequencing.Performed phylogenetic analyses using multiple programs.Complied with quarantine regulations.Cryopreserved bacterial and oomycete cultures.Instructed and managed undergraduate assistants.