Michael Holliday Email & Phone Number

Boston, Ma, Us

Cambridge, Massachusetts, United States

Cambridge, Massachusetts, United States

Cambridge, Massachusetts

South San Francisco, Ca

As a postdoctoral fellow in the Early Discovery Biochemistry department, my research has focused on developing a structural and mechanistic understanding of regulation and activation in a family of immune signaling proteins comprising CARD9 and CARD11. As part of these studies, I have determined the structures of numerous biologically relevant constructs and conformations, utilizing X-ray crystallography, Cryo-EM, and NMR spectroscopy. Leveraging the insight provided by these structures, I was able to structurally characterize distinct autoinhibition and activation mechanisms utilized by CARD9 and CARD11, including providing a structural rationale for disease-causing mutations in both proteins. Functional and structural characterization of these activation mechanisms was possible through extensive internal and external collaborations. In support of this and other projects, I conducted extensive construct design and generation, developed a range of protein purification protocols, and carried out biophysical and biochemical characterization

Greater Denver Area

As a graduate student in the Molecular Biology Department and the Structural Biology and Biochemistry Department, my doctoral research focused on using NMR and other biophysical techniques to characterize allosteric communication in the cyclophilin family of peptidyl-prolyl isomerases. I developed a novel method to map allosteric networks in proteins utilizing perturbations to local conformational dynamics and demonstrated a proof-of-concept in the model system Cyclophilin A. As part of these studies, I determined chemical shift assignments, functionally characterized, and compared conformational dynamics among multiple cyclophilin family members, allowing us to correlate altered dynamics with distinct aspects of the cyclophilin catalytic cycle. I additionally determined the structure of a previously uncharacterized thermophilic Cyclophilin, which allowed for identification of a novel substrate binding mechanism likely associated with its functionality at elevated temperatures. As part of these studies, I collaborated extensively with multiple computational labs.

Conducted research in the Department of Pediatrics, focused on revealing the role of PI3K in the development of obesity-related insulin resistance. I conducted research utilizing a range of techniques including flow cytometry, western blotting, IHC, qRT-PCR, primary cell culture from mouse and non-human primates, mouse physiology studies, and protein activity assays. Duties also included general lab management and maintenance of multiple mouse lines.

Hanover, Nh

Doctor Of Philosophy - Phd, Structural Biology

Master’S Degree, Engineering Physics

Bachelor’S Degree, Physics, Biochemistry