Michael Sennett Email & Phone Number

Seattle, Wa, Us

Seattle, Wa, Us

Seattle, Wa, Us

• Leading a team in the development of an experimental and analysis platform to identify therapeutics with favorable properties for manufacturability (e.g., thermal stability, titer, acid stability, and aggregation)• Leading a team to build an integrated experimental and informatics platform for rapid antigen generation, from DNA synthesis to antigen functional validation• Building a large language model for antibody protein sequences to improve antibody expression and yield• Wrote a program to identify covarying sites among antibody variable genes that require engineering and propose variant pairs to improve stability• Led a team to experimentally test de novo antibody designs and optimize those protein designs through affinity maturation and rational design via protein structural modeling • Led a team to build high-quality and diverse libraries of ML generated therapeutics for phage display display platform

Waltham, Ma, Us

• Wrote software to simulate evolution of proteins along a known phylogeny. The probabilistic model of evolution is based on the stability of a proteins 3D structure and principles of population genetics. This model can be used to test assumptions in evolutionary models and benchmark other programs.• Testing a machine learning program that performs ancestral protein reconstruction without the explicit assumptions made in model-based likelihood reconstructions in order to learn new evolutionary principles.

Waltham, Ma, Us

• I developed a computational workflow using bash and python to predict modern protein and DNA sequences given a probabilistic model of evolution. I then developed novel and informative metrics to asses the accuracy, bias, and precision of those protein predictions.• Developed a novel model-selection criteria to find most predictive hyperparameters for sequence inferences in probabilistic models. I then statistically analyzed sequence inferences to understand the effect of each model parameter and visualized the results to summarize key findings.• Identified the rate limiting steps for modern and ancestral Cryptosporidium lactate and malate dehydrogenases to study the evolution of enzyme mechanisms using spectrophotometric single- and multiple-turnover assays.• Resurrected ancestral enzymes and characterized their thermal stability and activity as a potential tool for protein engineering. I found enzymes that displayed high activity and stability, which are desirable traits for industrial applications.

Tarrytown, New York, Us

• Applied red-edge excitation spectroscopy to probe nanosecond dynamics of mAbs in the presence of co-solutes. • Biophysical characterization of mAbs for Investigational New Drug (IND) Applications. • Solely responsible for colloidal and conformational characterization of lead candidates in the protein biochemistry group for early investigational phase development.

Tarrytown, New York, Us

•Biophysical characterization of mAbs for Investigational New Drug (IND) Applications. •Solely responsible for colloidal and conformational characterization of lead candidates in the protein biochemistry group for early investigational phase development.• Developed a site-specific antibody conjugation method for fluorescence polarization binding assays.

University Park, Pa, Us

•Analyzed protein structure on the nanometer scale using single-molecule fluorescence resonance energy transfer. •Expressed and purified histone proteins and DNA to assemble nucleosomes for in vitro characterization.

Buffalo, Ny, Us

• Synthesized quantum dots for in situ production on a glass surface functionalized with TiO2 nanoparticles. Characterized the nanoparticle size and heterogeneity using UV-Vis spectroscopy.• Synthesized novel bi-functional phosphate-thiol molecule for quantum dot-TiO2 linkage to increase robustness in dye-sensitized solar cells. Resulted in hands on experience with organic column chromatography and phosphorus NMR.

Philadelphia, Pennsylvania, Us

• Handled input and filling of pharmacy prescriptions, as well as inventory maintenance to prevent customer loss. Helped to field customer questions and concerns by developing a rapport.

Buffalo, Ny, Us

• Synthesized organic amine ligands for coordination to Cu (II) Acetate and subsequent crystallization to mimic protein active site. Developing experience in area of crystal formation for x-ray crystallography.

Doctor Of Philosophy - Phd, Biochemistry And Biophysics

Master'S Degree, Biophysical Chemistry

Bachelor'S Degree, Chemistry And Biochemistry