Mohammad Abdullah Email & Phone Number

Newark, Nj, Us

Newark, Nj, Us

Playing a role in a research experiment and analyzing data to draw important conclusions and insights that further the field's scientific understanding. Examine the results presented both orally and in writing at a scientific conference. Focus on understating how the Amyloid Precursor Protein (APP) normally functions and how APP mutations linked to Alzheimer's disease affect APP's functionality in relation to the composition, destination, and purpose of APP-containing extracellular vesicles. We use biochemical, cell biology, and histological techniques with cellular and knock-in rat models of AD to achieve our research objectives.Essential responsibilities:Participates in the establishment of performance standards, as well as in the selection of methodology and instrumentation.Carries out various technical and research operations relating to investigative activities of the laboratory.participates in organizing and conducting specialized and research activities as it relates to the function of the amyloid precursor protein and its role in extracellular vesicles.participate with the instructing and training of students, fellows and technicians in various techniques and procedures to develop the skills necessary to carry out assignments in the laboratory assistants.Contributes to the maintenance of the rate colony.Coordinate, supervise and evaluate the work of non professional staff.

Rochester, Minnesota, Us

In this role, I presented experimental findings at scientific conferences and examined data to develop significant conclusions and insights, contributing to scientific knowledge and progress. Also, I utilize microscopy techniques to observe cellular architecture and interactions, ensuring efficient processes.Key achievements and contributions during this tenure include:★ I conducted assays, screens, lead optimization, and mechanistic investigations to analyze and investigate the interactions of small compounds, resulting in a comprehensive understanding of their impacts and potential applications.★ I developed pharmacological techniques for changing new targets, studied immune cells and cellular models of immunological and neurological disorders, and evaluated immune cells from autoimmune patients.★ I ensured compliance with safety standards, quality assurance requirements, and SOPs by thoroughly examining and understanding IACUC procedures to respect regulatory and ethical standards to promote safety and quality assurance.★ I created EV isolation and characterization methods, specifically exosomes, macrovesicles, and large vesicles, to investigate tau and amyloid beta-related neurodegenerative diseases linked with proteins, enhancing comprehension of pathologies.

Boston, Ma, Us

While working in this role, I conducted experiments with precision, analyzed and interpreted data, and drew meaningful conclusions. Also, I ensured the safety and welfare of animals during research procedures while maintaining the functionality and viability of cell lines and primary cells. Additionally, I contributed to research endeavors and made valuable contributions to the scientific community.Key achievements and contributions during this tenure include:★ I leveraged online research tools and extensive literature searches to help with experiment design, optimization, and technological issues, achieving predictable results with high-quality data.★ I consistently produced trustworthy data by completing procedures with accuracy and minimum help, assessing and interpreting complex experiment findings, and making meaningful and important conclusions.★ I collected data from various experiments and programs and created visually appealing PowerPoint presentations to effectively communicate results to colleagues, highlighting key findings and outlining future research directions.★ I designed and conducted in vitro experiments for compound screening with minimal supervision to advance research goals and objectives, ultimately driving the progress of research goals and objectives forward.

During this position, I collected and processed human fluids, including cerebrospinal, serum, and cervicovaginal fluids, to support Alzheimer's disease research. Also, I analyzed to explore the potential of serum flotillin levels as a biomarker for Alzheimer's disease by examining their association with mean cortical PiB retention levels.Key achievements and contributions during this tenure include:★ I performed biochemical tests such as SDS-PAGE, western blotting, and ELISA to study morphological characteristics and functions of extracellular vesicles (EVs) from cultured cells and mice brain tissue, resulting in major scientific discoveries.★ I enhanced research efficiency by depleting cholesterol, treating it with medications, and monitoring cholesterol levels by utilizing different ways to study cell impacts and prospective therapeutic uses.★ I employed conventional techniques to generate primary astrocytes, microglia, and neuron cells, supporting neuroscience investigations and innovating therapeutic interventions, elevating research efficiency and precision.★ I identified disease indicators in human cerebrospinal fluid (CSF) and cervical-vaginal fluid (CVF) samples from individuals with Alzheimer's disease (AD), non-AD, and vascular dementia.

In this role, I isolated pure exosome samples through ultracentrifugation and sucrose gradient fractionation for further analysis. Additionally, I cultivated primary astrocytes and microglia cells from rat brains and evaluated their viability with cell survival assays to identify potential therapies for neurological diseases.Key achievements and contributions during this tenure include: ★ I developed and improved a co-culture method to study neuronal-astrocyte interactions in memory, learning, neuroinflammation, and neurodegenerative disorders.★ I produced genetically modified mice, including ApoE3, ApoE4, ApoE-knockout, and wild-type mice to investigate the functions and mechanisms of ApoE in various disease models and situations for effective research methodologies.★ I analyzed EV proteins, quantified targeted compounds, and performed SDS-PAGE, western blotting, and ELISA tests on extracellular vesicles (EVs) from cell-conditioned medium and cell lysates. Complex research challenges.★ I established primary cultures of astrocytes, microglia, and neurons using standard methods, providing a valuable tool for studying the properties and functions of cells and interactions in various physiological and pathological conditions.

Doctor Of Philosophy In Medical Science, Medical Science

Master'S Degree, Entomology And Insect Biotechnology, Natural Product Chemistry

Zoology/Animal Biology