CMC API Development

Back-end development:• C# and SQL

Big data migration:• C# and SQLMicrosoft Azure:• Function Apps• SendGrid emails• Notification Hub (push messages)Power BI:• Customer analytics reports

G Protein-Coupled Receptor signaling. Professor Michelle Glass, Department of Pharmacology and Clinical Pharmacology, The University of Auckland, New Zealand.• Cell-based assays: BRET, AlphaScreen, and GPCR trafficking.• High-throughput microscopy: Immunocytochemistry followed by high-content screening.• In vitro biochemistry: GPCR radioligand binding assays.• Protein expression: Mammalian cells.• Protein purification: Membrane preparation of GPCRs.

The composition and structure of the Cydia pomonella granulosis virus envelope. Associate Professor Peter Metcalf, School of Biological Sciences, The University of Auckland, New Zealand.• Fermentation/Protein expression: E. coli.• Protein purification: Centrifugation and HPLC.• Biophysical characterization: SEC-MALLS and circular dichroism.• Mass spectrometry.• Structural biology: Electron microscopy, cryo-EM, and X-ray crystallography.• Protein engineering: Cloning, site-directed mutagenesis, and deletion.• In vitro biochemistry: SDS-PAGE.

The antiviral mechanism of OASL. Professor Rune Hartmann, Centre for Structural Biology, Department of Molecular Biology and Genetics, Aarhus University, Denmark.• Fermentation/Protein expression: E. coli, insect, and mammalian cells.• Protein purification: Centrifugation, filtration, precipitation, and HPLC.• Enzymatic assays: Synthetase (³²P) and ATPase assays.• In vitro biochemistry: Native PAGE and western blot.• Biophysical characterization: Analytical SEC and microscale thermophoresis.• Structural biology: X-ray crystallography and SAXS.• Protein engineering: Cloning, fusion, site-directed mutagenesis, and deletion.• Cell-based assays: immuno precipitation, RT-qPCR, stable transfection, reporter gene expression, and viral infection assays (live-virus class 2).

Theoretical exercises:• Immunology; four semesters, responsible for developing assignments and parts of the exam.• General biochemistry; two semesters.Laboratory exercises:• General molecular biology; two semesters.

β-arrestin recruitment to Cannabinoid Receptors CB1 and CB2Queenstown Research Week, 15th of August 2014, Queenstown, New Zealand, oral presentation.The crystal structure of the antiviral OASL, Molecular Biology and Genetics annual meeting, 15th of August 2014, Aarhus, Denmark, invited speaker, oral presentation.The crystal structure of OASL, Danish Society of Immunology annual meeting, 2nd of May 2014, Odense, Denmark, oral presentation.Regulation of gene expression by OASL, ARCII meeting, 21st of November 2013, Aarhus, Denmark, oral presentation.Roles of OASL during viral infection, ARCII meeting, 7th of March 2013, Aarhus Denmark, oral presentation.Mapping the active site and investigating the polyI:C inducibility of the human 120 kDa 2’-5’ Oligoadenylate Synthetase 3, 9th joint meeting of ICS-ISICR Cytokines and Interferons, 10th to the 12th of October 2011, Naples, Italy, and the ICS-ISICR satellite symposium: Interferon Stimulated Genes and Their Protein Products-Personalizing for Molecular Medicine, 13th to the 14th of October, Prato, Italy, poster session.

Personal mentor for students with special needs at the Department for Molecular Biology and Genetics.