*Identified novel human protein isoform, characterized by protein expressionand over-expression in cell lines, sub-cellular localization, and activity fromhuman and mouse cell lines and brain tissues.* Characterized multiple in vivo and in vitro models of schizophrenia,astrogliosis, glioblastoma, systemic lupus erythematosus, stroke via proteinbiochemical and gene expression (qRT-PCR) analyses.* Cloned a library of shRNA constructs and produced lentivirus for multipleknockdown studies in rodent neurons and glial cells. Lentivirus were titeredand measured by flow cytometry.* Constructed gene targeting vectors using homologous recombination toclone large fragments of DNA into BACs. Further validated targeting vectorsvia quantitative genomic copy number PCR assays. Characterized resulting invivo and in vitro models via qRT-PCR.* Cloned shRNA using a lentiviral-based system. Validated knockdown ofmore than 20 target genes by HEK293T transfection, collecting and titteringviral stock, transducing mouse neurons, and measure gene expression byqRT-PCR.