Munan Shaik Email & Phone Number

Boston, Massachusetts, United States

Design and production of high quality protein reagents and x-ray crystallography/cryoEM structures that drive drug discovery and target validation projects.

Natick, Massachusetts, United States

Structure based rational design of mAb, bispecific and multispecific antibody for stability, affinity and developability.Design, Expression and purification of antigens/protein for immunization and binding studyMammalian display for affinity improvement.Bioluminate, MOE based library design and golden gate cloning platform for large scale library generationBiochemical binding study by octet

Cambridge, Massachusetts, United States

Designed and produce recombinant proteins in bacteria, insect and mammalian system to meet project needs in assays and screenings, biophysics characterization, structural and MOA studies.Determine X-ray crystallography and cryoEM structure of targets with small molecule.Managed internal FTE and CRO FTE/FFS resources to deliver high quality protein reagents and x-ray crystallography/cryoEM structures that drive drug discovery and target validation projects.Enabled new… Show more Designed and produce recombinant proteins in bacteria, insect and mammalian system to meet project needs in assays and screenings, biophysics characterization, structural and MOA studies.Determine X-ray crystallography and cryoEM structure of targets with small molecule.Managed internal FTE and CRO FTE/FFS resources to deliver high quality protein reagents and x-ray crystallography/cryoEM structures that drive drug discovery and target validation projects.Enabled new drug target by producing protein and X-ray structure. Show less

Cambridge, Massachusetts, United States

Memphis, Tn

Role and responsibilities:1. Lead research projects from Gene to Structure (X-tal, CryoEM) or gene to protein (High throughput assay) to support continuing research and development at St Jude Children's Research Hospital.2. Support research and development of projects from St. Jude members (Faculties).3. Establishment of world latest cutting edge facilities for Protein Technology as a member of the Protein Technology Center in Department of Structural Biology, St Jude… Show more Role and responsibilities:1. Lead research projects from Gene to Structure (X-tal, CryoEM) or gene to protein (High throughput assay) to support continuing research and development at St Jude Children's Research Hospital.2. Support research and development of projects from St. Jude members (Faculties).3. Establishment of world latest cutting edge facilities for Protein Technology as a member of the Protein Technology Center in Department of Structural Biology, St Jude Children's Research HospitalOngoing Project:1. Structural of Human Mitochondrial ABC B-family transporter by cryo-EM. Refined to 3.7 A (Apo, inward-facing) and substrate and ATP analog bound form.2. Structure-based characterization of B-cell receptor CD19 in complex with the antibody for better development of CAR-T cell therapy for cancer. Stable cell lines made for CD19 expression.3. Structural study of Mitochondrial transporter of one-carbon metabolism pathways. Transporter purified and is in a crystallization trial (LCP). 4. Expressed in Insect cell system and purified DDB1 (DNA binding protein) and CRBN (Cereblon) and various IKZF (Ikaros Zing Finger) protein for E3 ligase mediated Protac project.5. Optimization, Expression, and purification of the different membrane and secreted proteins in the Insect cell expression system and Mammalian cells expression system for structural and functional study. Show less

Boston, Massachusetts, United States

Boston, Massachusetts

Role and responsibilities:1. Lead research projects in the group from Gene to Structure (X-tal, CryoEM) to support continuing research and development of HIV1 vaccine discovery and HIV1 biology.2. Developed membrane protein expression and purification and cryoEM structure determination pipeline in the group.3. Developed cell-based, fluorescence-based assay in the group. 4. Supervised technician and provide training to the junior fellow and graduate student.Achievements… Show more Role and responsibilities:1. Lead research projects in the group from Gene to Structure (X-tal, CryoEM) to support continuing research and development of HIV1 vaccine discovery and HIV1 biology.2. Developed membrane protein expression and purification and cryoEM structure determination pipeline in the group.3. Developed cell-based, fluorescence-based assay in the group. 4. Supervised technician and provide training to the junior fellow and graduate student.Achievements and experiences:1. Structural Basis of Transmembrane Coupling of the HIV-1 Envelope Glycoprotein (structure of Cytoplasmic domain and Transmembrane domain) (published in Nature Comm, 2020).2. High-resolution Cryo-EM structure of CCR5 (GPCR) in complex with HIV1 envelope glycoprotein and Human T-cell surface marker (CD4). (Published in Nature, 2019).3. Structure of the membrane-proximal external region of HIV-1 envelope glycoprotein, (Published in PNAS, 2018).4. Optimize GPCR (CCR5 and CXCR4) expression and purification from mammalian expression system.5. Developed and mammalian expression and purification protocol for a variety of HIV-1 GP120, GP140 and GP160 construct from different isolates.6. Expressed and purified many HIV-1 broadly neutralizing and non-neutralizing antibody (IgG and Fab) from a hybridoma and transient transfection/ stable cell lines. 7. Analyzed a variety of HIV-1 antigen constructs binding affinity with a different antibody/small molecule compounds by ELISA, SPR, Octate.8. Developed the FACS protocol for cell surface expression of GPCR (CCR5, CXCR4) and formation of cell surface complex with CD4 and HIV-1 envelope. Technical expertise:Cryo-Electron microscopy. Molecular cloning and mutagenesis, Mammalian Cell Culture, Transfection, GPCR expression and purificationSPR, FACS, ELISA, Negative Staining Electron Microscopy. IgG, FAB, Clasp Fv antibody design, cloning, expression, refolding purification and characterization Show less

Calgary, Canada

Achievements and experiences:1. Expressed in E. coli, purified, crystallized and determined the X-ray crystallography structure of Norovirus polymerase (NVPol) in complex with duplex RNA (Primer and template) and nucleotide, and putative inhibitor.2. Structure based design of small molecule for RNA dependent RNA virus polymerase (Norovirus, HCV). 3. Developed Fluorescence-based Polymerase inhibition assay for small molecule inhibitor.3. Determined eight complex… Show more Achievements and experiences:1. Expressed in E. coli, purified, crystallized and determined the X-ray crystallography structure of Norovirus polymerase (NVPol) in complex with duplex RNA (Primer and template) and nucleotide, and putative inhibitor.2. Structure based design of small molecule for RNA dependent RNA virus polymerase (Norovirus, HCV). 3. Developed Fluorescence-based Polymerase inhibition assay for small molecule inhibitor.3. Determined eight complex structures of NVPol-RNA with small molecule inhibitor by X-ray crystallography.4. X-ray data Collection, structure determination and refinement of the structure of FAB in complex with putative carbohydrate from Mycobacterium tuberculosis.Technical Expertise: Expression, purification of recombinant protein,AKTA FPLC, Biorad purification systemPolymerase - small molecule binding analysisCrystallization of polymerase with inhibitor and determine the structure.Structure refinement.DSF and fluorencense techniques for protein-ligand binding study,Purification of FAB, MAbX-ray crystallography of FAB, MAb with different drug target. Show less

Grenoble, France

Achievements and Experiences:1. Design Constructs, cloning, optimize expression, and purification of Minor pilin (RrgC) protein from Streptococcus pneumonia. Rational site-directed mutagenesis for successful crystallization, and used Single anomalous X-ray diffraction (SAD) techniques to determine the structure. Employed SPR to explored the binding affinity to other pilin and in-vivo knock-out mutagenesis to address the correct sortase responsible for linking of the basal pilin to the… Show more Achievements and Experiences:1. Design Constructs, cloning, optimize expression, and purification of Minor pilin (RrgC) protein from Streptococcus pneumonia. Rational site-directed mutagenesis for successful crystallization, and used Single anomalous X-ray diffraction (SAD) techniques to determine the structure. Employed SPR to explored the binding affinity to other pilin and in-vivo knock-out mutagenesis to address the correct sortase responsible for linking of the basal pilin to the cell wall.2. Molecular Cloning, expression and purification of Major pilin (PitB) and responsible Sortase (SrtG1) protein from Type-II pilius of Streptococcus pneumonia. Crystalized both of them and Rational site-directed mutagenesis based introduction of two extra Methionine to PitB to phase by SAD method and SrtG1 structure solved by Molecular Replacement (MR). Protein-protein docking and Negative stain Electron Microscopy (EM) used to functional residues for linking pilus and structural details of how SrtG1 catalyze the protein polymerization reaction.3. Development and Optimization of Cell-free expression protocol of ComEC (integral membrane) membrane protein from Type-IV pilus. Detergent screening and optimization of purification, crystallization in LCP, Negative stain EM.Technical Expertise:Molecular Cloning, Rational design for site-directed mutagenesis,Expression, purification of recombinant protein,AKTA FPLC, Agilant HPLC, Cell free expression of membrane proteinsDetergent screening and purification of membrane protein,Protein Crystallization, HTS screening, LCP crystallization of membrane proteins,X-ray crystallography, SAD, MAD phasing, Structure refinement.SPR and fluorencense techniques for protein-protein and protein-ligand binding study Show less

Padua, Veneto, Italy

Achievements and Experiences:1. Design Constructs, cloning, optimize expression, and purification of Petidoglycan Deacetylase (virulence factor) from Helicobacter pylori. Determined X-ray crystallography structure, analyze enzyme activity with the different putative substrate. Using X-ray fluorescence scan determined the active site metal and quantum chemical methods calculate the free energy for all the possible metals in the active site. QCMM calculations used to determine the reaction… Show more Achievements and Experiences:1. Design Constructs, cloning, optimize expression, and purification of Petidoglycan Deacetylase (virulence factor) from Helicobacter pylori. Determined X-ray crystallography structure, analyze enzyme activity with the different putative substrate. Using X-ray fluorescence scan determined the active site metal and quantum chemical methods calculate the free energy for all the possible metals in the active site. QCMM calculations used to determine the reaction mechanism for better designing of the mechanistic-based inhibitor. 2. Molecular cloning, optimize expression, purification crystallization, and determined the X-ray crystallography structure of ADP-L-glycero-D-manno-heptose-6-epimerase from H. pylori lipopolysaccharide (LPS) biosynthetic pathways. 3. Molecular cloning, optimize expression, purification crystallization, and determined the X-ray crystallography structure of Nickel transporter (CeuE) from H. pylori lipopolysaccharide (LPS) biosynthetic pathways. Mutagenesis, Co-crystallization with His-Ni complex, and SPR data generated to confirm the functional role in H. pylori.Technical Expertise:Molecular Cloning, Site-directed mutagenesis,Expression, purification of recombinant protein,AKTA FPLC, Agilent HPLC, Biorad purification systemProtein Crystallization, HTS screening, X-ray crystallography, MR, SAD, MAD phasing, Structure refinement,CD, DLS, ITC, SPR and fluorencense techniques for protein-protein and protein-ligand binding study Show less

Doctorate, Protein Crystallography, Structural Biology

Xvii Bca/Ccp4 Summer School In Macromolecular Crystallography, Macromolecular Crystallography

Summer Course, Fermentation Engineering And Microarray Analysis

Master’S Degree, Biotechnology And Genetic Engineering