Sheetal Kumar Email & Phone Number

Boston, Massachusetts, United States

Greater Boston Area

Cambridge, Usa

Manage in vitro predictive safety profiling assays across several therapeutic areas and modalities (small molecule, biologics, siRNA, PROTAC). Drive wet lab efforts and developed a variety of cell-based models (iPSC, primary cell, & 3D) for novel assays to investigate drug safety issues. Interface with external partners and CRO’s to establish robust experimental platforms and techniques for translational preclinical safety assessments.• Independently worked to establish a human iPSC-based blood brain barrier in vitro model for predictive safety, presented findings at the 13th International Conference on Cerebral Vascular Biology• Manage a panel of early screening assays to identify safety liabilities for Amgen pipeline projects• Co-led the RNAseq toxicogenomics investigations with senior scientists to interpret results and de-risk clinical safety findings resulting in nomination of “EVENITY” into market• Guided postdoctoral project to implicate a novel target in hepatic lipid accumulation and validated hypotheses using various molecular and pharmacological techniques (CRISPR, high content imaging) • Developed and optimized the Amgen-CBSS RNA extraction platform for multiple tissue sequencing• Function as a senior bench scientist, applying an array of cell and molecular biology techniques (qPCR, RNA seq. & click-chemistry) to derive insight into molecular pathways as well as provided mentorship and training to associates within the team• Interface collaboratively with groups within Amgen and external CRO’s to establish robust experimental platforms and techniques to further research goals• Perform data analysis and presented key finding at department and project team meetings

Greater Boston Area

Supported multiple pre-clinical programs in Heart Failure, Vascular Inflammation and Pulmonary Arterial Hypertension (PAH); proficiency in techniques include:• Plate based pharmacological assays including ‘FLIPR’ and ‘Click-IT’ assays to assess target engagement, proliferation and cell viability to identify small molecule leads.• Extensive mammalian cell culture including cardiomyocytes, smooth muscle cells, endothelial cells and immune cells• Extensive biochemical and molecular biology including: Immuno-fluorescence, quantitative RT-PCR and Western blotting from tissues and cells• Elucidated novel bio-markers using cytokine arrays and ELISA• Actively presented data and findings at group and line meetings

Bethesda, Maryland, Usa

Investigated the role of RCC1-dependent activation of Ran▪GTP-regulated nuclear-cytoplasmic transport, which accelerates cell cycle and DNA damage response.• Performed extensive DNA cloning, bacterial/mammalian protein expression and purification• Utilized western blotting and imaging techniques to elucidate transport mechanisms• Designed new workflow methodology to allow for multitasking which kept project on track

Cambridge, United Kingdom

Project 1: Regulation of OGG1 in Vascular disease.• Investigating the role of role of SIRT-1 in vascular cell senescence and atherosclerosis• Tissue culture lab manager-responsible for maintaining the smooth running of the facility. Project 2: DNA Damage in Atherosclerosis, its association with Vulnerable Plaque• Investigating the role of SIRT-1 in vascular cell senescence and atherosclerosis• Tissue culture lab manager-responsible Project 3: The Role of Sirtuins in Vascular Smooth Muscle Cells• Investigating the role of role of SIRT-1 in vascular cell senescence and atherosclerosis• Tissue culture lab manager-responsible for maintaining the smooth running of the facility.

Project: RNase L Activation• Expressed and purified enzymes in bacteria and Baculo viruses. • Amplify viral stock for large scale enzyme production for functional & structural analysis like rRNA cleavage, X-Ray crystallography & NMR.• Designed primers for sequencing, cloned and set up PCR reactions for DNA fragment amplification and restriction site insertion, • Analyzed DNA by agarose gel electrophoresis.• Performed enzymatic synthesis of 2’-5’ linked oligoadenylates and their purification using HPLC.• Performed High Throughput Screening for candidate drug development using in-vitro Fluorescence Resonance Energy Transfer (FRET) assay• Worked extensively with mammalian and insect cell culture, keeping stock of various cell lines in a group of nearly 15 scientists with Cancer Biology Department of Cleveland Clinic Foundation.• Responsible for shipping reagents (certified in infectious substance shipping methods), ordering chemicals and general lab maintenance.

Project: Effects of Statins on the treatment of Ovarian Cancer.• Postulated and proved the molecular mechanism for Statin-induced Apoptosis in Ovarian Cancer Cells Determined cell apoptosis using electrophoresis/western blot techniques. • Findings were supported through Flow-cytometry, Immuno-precipitation, Western blot and Enzyme-Linked Immuno Sorbent Assay, (ELISA)• Managed chemical purchases, requisites, and material safety data sheets, (MSDS)• Collaborated with Department of Cancer biology, Cleveland Clinic Foundation

Master'S Degree, Bioanalytical & Clinical Chemistry, 3.9/4.0

Statins induce apoptosis in Ovarian Cancer Cells

Master'S Degree, Chemistry, 3.6/4.0

Activities and Societies: Group conference-meeting organizer Group Chemical Purchaser, MSDS etc...Pyrazinamide Studies

Bachelor'S Degree, Chemistry, 3.8/4.0

•Graduated Magna cum laude

Certificate, Level 3 Line Management

Activities and Societies: Concentration in organization, communication, delegation, problem resolving, team management