• Established involvement of MMP-2 and MMP-9 in severe brain damage in ischemic stroke with hyperthermia.• Successfully cloned and expressed TIMP-1 gene from ischemic hemisphere of rat brain tissue in an attempt to use recombinant rat brain TIMP-1 for attenuating hemorrhagic complications and enhancing stroke recovery.

• Accomplished the purification and characterization of a novel & long sought AMPK kinase (AMPKK) from rat heart tissue. AMPKK is an upstream kinase of AMPK and regulates functional activities of AMPK. AMPK is considered the master switch of all cellular and whole body energy metabolisms in mammals.• Established strategies to express and purify functional recombinant 3-KAT (3-ketoacyl-CoA thiolase) in E. coli and insect cells.• Utilized purified functional 3-KAT for assessing the… Show more • Accomplished the purification and characterization of a novel & long sought AMPK kinase (AMPKK) from rat heart tissue. AMPKK is an upstream kinase of AMPK and regulates functional activities of AMPK. AMPK is considered the master switch of all cellular and whole body energy metabolisms in mammals.• Established strategies to express and purify functional recombinant 3-KAT (3-ketoacyl-CoA thiolase) in E. coli and insect cells.• Utilized purified functional 3-KAT for assessing the beneficial effects of Trimetazidine and Ranolazine (anti-anginal drugs) on mitochondrial substrate oxidation and cardiac function by selectively inhibiting long chain 3-KAT. Show less

• Cloned, expressed and purified human triacylglycerol hydrolase (hTGH) in E. coli.• Established baculovirus expression system for making functional hTGH in insect cells (Sf-9) and other mammalian cell lines (MacArdle RH7777, Cos7 cells, 3T3, 3T3L1 etc.).• Successfully produced and purified polyclonal rabbit anti-hTGHs antibodies.• Identified catalytic residues of hTGH by site-directed mutagenesis.• Performed In vivo studies of lipid metabolism on transgenic mice (C57 black)… Show more • Cloned, expressed and purified human triacylglycerol hydrolase (hTGH) in E. coli.• Established baculovirus expression system for making functional hTGH in insect cells (Sf-9) and other mammalian cell lines (MacArdle RH7777, Cos7 cells, 3T3, 3T3L1 etc.).• Successfully produced and purified polyclonal rabbit anti-hTGHs antibodies.• Identified catalytic residues of hTGH by site-directed mutagenesis.• Performed In vivo studies of lipid metabolism on transgenic mice (C57 black) expressing hTGH under metallothionein (MT) promoter. Show less

Biotechnological application of cellulose binding domain.Constructed different recombinant expression vectors for producing fusion proteins of cellulose binding proteins with different enzymes such as amylase, protease and lipase for use in detergent.Purified expressed fusion proteins through open column and FPLC purification system.