Natalia Pardo Lorente Email & Phone Number

Barcelona, Ct, Es

Barcelona, Catalonia, Spain

Barcelona, Catalonia, Spain

During this time, I have completed my main research project resulting in the publication of my first first-author scientific publication (available at: https://doi.org/10.1101/2023.06.01.543193), which is currently under peer review. In addition, I have written a scientific review about the canonical and non-canonical roles of the metabolic enzyme MTHFD2 in health and disease. On the other hand, I have also collaborated in other projects related to the characterisation of novel roles of metabolic enzymes in the nucleus of cancer cells, both experimentally, performing experiments, or analysing high-throughout data (RNA-sequencing, CRISPR-genetic screening, high-throughput microscopy). Besides, I also designed a project that emerged from a machine learning-based clinical oncology data analysis, with a potent translational output in the cancer screening and early diagnosis field, and asked for an innovation proof-of-concept grant.Competences:• Deep knowledge in the fields of cancer research, epigenetics, metabolism and gene regulation• Experience analysing and interpreting omics datasets from cancer patients and cancer cell lines• Programming with bash, R and Python • Use of the HPC cluster• Retrieve datasets from public repositories• Project management of 2 main projects and collaborations with 8 projects, meeting tight deadlines• Critical thinking and decision-making ability• Teamwork in an international dynamic environment• Oral communication, participating in international conferences• Written communication: scientific articles and reviews• Familiarity with writing scientific project proposals addressed to funders or clinical research ethics committeesConferences:• Oral Communication in the Asilomar Chromatin, Chromosomes & Epigenetics Conference (2023), where I won the best presentation award.

Barcelona, Catalonia, Spain

In the Sdelci lab, we are interested in the interplay between epigenetics and metabolism in cancer, where my project aimed to decipher novel roles of a metabolic enzyme that is a promising cancer therapeutic target. I discovered that the nuclear form of this enzyme alters their epigenetic regulation of cancer cells and impairs cell division. ➛Granted a Boehringer Ingelheim Fonds (BIF) PhD fellowship (2020-2023).• Deep knowledge in the fields of cancer research, epigenetics, metabolism and gene regulation• Experience analysing and interpreting >10 omics datasets from patients and cancer cell lines: - genomics, transcriptomics, epigenomics and proteomics - whole-genome genetic screenings - programming with bash, R and Python - use of the HPC cluster - retrieve datasets from public repositories• High expertise performing laboratory techniques: - NGS library preparation - whole-genome genetic screenings - CRISPR genetic engineering - molecular biology, protein assays, microbiology and virus handling - cell culture and cellular assays - microscopy and flow cytometry• Other relevant competences acquired: - project management of >4 projects, meeting tight deadlines, and reporting back to funders - critical thinking and decision-making ability, designing research plans and methods, and troubleshooting experiments - teamwork in an international dynamic environment - oral communication, participating in >6 national and international conferences - written communication: 3 scientific articles and 1 review - leadership, by directly supervising master and early PhD students Conferences:• Oral Communication in the Elevator Pitch of the SEBBM (2022), the 23rd MetNet National Annual Meeting (2022) and the III Interdisciplinary Congress on Human Genetics (2021)• Poster Presentation in the EACR conference "Cancer Metabolism" (2022), Cancer Metabolism Showcase (2022) and the EMBL conference “Metabolism Meets Epigenetics” (2021)

Cambridge, United Kingdom

Samarajiwa Lab: Computational Biology, Regulatory Genomics, Data Science. www.samarajiwa-lab.org/ Tutors: Dr. Shamith Samarajiwa and Dr. Sakari VanharantaMaster Project: I determined the role of two transcription factors in metastatic clear cell renal carcinoma (ccRCC) by analysing RNA-sequencing and ChIP-sequencing data from metastatic renal cancer cell lines. This thorough characterisation resulted in a better understanding of the altered biological pathways that play a key role in ccRCC tumor development.➛ Poster presentation in the EMBL conference “Perspectives in Translational Medicine” (2019).• Programming in R and bash• Pre-processing of RNA- and ChIP-seq data: quality check, trimming and alignment• RNA-seq pipeline: counting and identification of differentially expressed genes• ChIP-seq pipeline: peak calling and annotation to identify transcription factor binding sites and motif analysis• Integrated the differentially expressed genes with the transcription factor binding sites to identify the direct target genes of both transcription factors• Carried out functional downstream analysis to decipher their contribution in ccRCC• Knowledge about the genetic and metabolic hallmarks of clear cell renal carcinoma, and how the dysregulation of transcriptional networks can drive tumor development, which is key in the discovery of novel therapeutic targets

Valencia, Valencian Community, Spain

Hematology and Hemotherapy research group – Genetics UnitTutor and Researcher: Dr. José Cervera Zamora and Dr. Mariam Ibañez Company➛ Fellowship from the Spanish Association Against Cancer (AECC) for a Laboratory Internship during the Academic Course 2017-18Bachelor Project: I performed a molecular characterisation of hematologic neoplasias by means of Next Generation Sequencing (NGS). I evaluated the frequency of germline pathogenic variants in patients with Acute Myeloid Leukemia and Myelodysplastic Syndrome by using a custom multi-gene NGS panel. I concluded that the frequency of pathogenic germline mutations was higher than expected (~15%).➛ Prize to the best Bachelor thesis in the ETSIAMN – UPV (2018)➛ Oral Communication in the National Congress of Hematology and Hemotherapy (SEHH,SETH)• Used a targeted multi-gene NGS panel including genes related to hereditary malignant hematologic disorders to test DNA samples from a cohort of ~40 patients• Analysed, categorised and interpreted ~60 genetic variants detected using the ACMG guidelines• Handled clinical and biological databases (ClinVar, COSMIC, VarSome and dbSNP) and in silico biological predictors (PolyPhen, SIFT, MutationTaster, Human Splicing Finder and SPANR) • Participated in the preparation of genomic DNA libraries for NGS and completed a course of MiSeq (Illumina)• Validated the pathogenic variants using Sanger sequencing• Knowledge about the genetic landscape of hematologic cancers and how patients with hematologic malignancies are diagnosed and managed

Vienna, Austria

“Signaling Pathways” research groupTutor and Researcher: Dr. Thomas CzernyErasmus+ internship• Detected and analysed cellular stress toxicity upon treating cells with 4 heavy metals as part of a project that ended up in a scientific publication• Experience in several molecular biology and cell culture techniques

Valencia, Valencian Community, Spain

Hematology and Hemotherapy research group – Genetics UnitTutor and Researcher: Dr. José Cervera Zamora • Cytogenetics: analysed FISH and karyotypes to identify numeric and structural chromosomic alterations, and also helped in the preparation of karyotypes• Molecular Biology: performed PCR, electrophoresis and Sanger sequencing, and analysed and interpreted the results

Master Of Philosophy - Mphil, Genomic Medicine, Distinction

Activities and Societies: Granted a “la Caixa” scholarship for postgraduate studies in European universities (2018-2019) Modules: •.

Doctor Of Philosophy - Phd, Biomedicine

Activities and Societies: Participation in PRBB (Barcelona Biomedical Research Park) Open Day and in talks for secondary school students.

Master'S Degree, Bioinformatics And Biostatistics

I have become highly proficient in the field of bioinformatics, acquiring experience in several programming languages (R, Python, bash.

Bsc, Biotechnology, Graduated With Honours

Activities and Societies: Athletics Modules: • Basic Training: Cell Biology, Genetics, Microbiology, Physiology, Chemistry, Physics.