Nathan Lassig Email & Phone Number

• Have consistently received rave reviews from superiors and students• According to supervisor, I have “done the work of three”

• Designed and created a versatile plasmid that was far superior to the lab’s standard plasmid (This became the most used plasmid in my lab)• Used PCR to fuse many different genes in various attempts to create, de novo, a straight, stable, protein-based filament of monodisperse and controllable length• Developed in vivo expression and purification processes for E. coli chaperones IbpA and IbpB• Optimized expression of actin in two cell-free protein expression systems based on E… Show more • Designed and created a versatile plasmid that was far superior to the lab’s standard plasmid (This became the most used plasmid in my lab)• Used PCR to fuse many different genes in various attempts to create, de novo, a straight, stable, protein-based filament of monodisperse and controllable length• Developed in vivo expression and purification processes for E. coli chaperones IbpA and IbpB• Optimized expression of actin in two cell-free protein expression systems based on E. coli lysates• Demonstrated significant improvements in actin expression and solubility as catalyzed by IbpB • Became proficient at purification and analysis of expressed actin Show less

• Created and optimized synthesis of MC-214, a precursor to a cobalamin bioconjugate potentially useful in chemotherapy• Used flow cytometry to do numerous cobalamin uptake studies in various human and murine cell lines and modeled results

Ms, Bioengineering

Bs, Bioengineering