Nicholas Chambers Email & Phone Number

Morrisville, North Carolina, United States

My responsibilities as a Research Associate in the Analytical Department at DMC Biotechnologies are as follows:● Adherence to in-house SOPs during the preparation and analysis of bacterial metabolite samples using HPLC, UPLC, LC-MS, and UV-Vis spectophotometry.● Preparation of standard materials, moblie phase, and other reagents needed to carry out various SOPs as needed.● Maintenance, monitoring, and repair as needed of Waters™ Liquid Chromatography instruments to ensure consistent functioning for day-to-day testing schedules.● Development of qualified HPLC, UPLC, and LC-MS methods for quantifying new target metabolites or products of bacterial fermentation depending on upstream project goals.● Development of new SOPs for sample preparation and handling as new products and metabolites are added to meet research project goals.● Implementation and use of LabGuru ELN software to document all experimental sample preparations, method development experiments, and stock preparations according to in-house SOPs.● Implementation and use of LabGuru ELN software to apply statistical analysis and report data for samples coming from upstream processes.● Communication within an interdisciplinary, multi-department, multi-site team to ensure that data turn-around for the Molecular Biology/Genomics and Fermentation departments occurs quickly to allow for data-driven project decisions within those departments.● Alignment of personal day-to-day activities with OKRs set forth by Business Development and the analytical department supervisor to meet corporate KPIs for attracting investors.● Training of other research associates on procedures used in the Analytical Department.

Raleigh, North Carolina, United States

My responsibilities as a Quality Technician at Mallinckrodt Pharmaceuticals included:● Adherence to cGLP and cGMP SOPs during the sample preparation and analysis of in-process and finished lot materials involving the acetaminophen manufacturing process.● Use of BPCS and Mallinckrodt LIMS systems to log, receive, manage, and create data reports on batches of raw materials, in-process materials, and finished goods.● Use of Chromeleon chromatography software to operate multiple HPLC instruments needed for various tests throughout the shift, integrate results after completed tests, and generate reports on tested materials.● Preparation of laboratory reagents, solutions, and standards according to SOP daily as needed.● Creating Trackwise reports in the event of any process materials being out of control and carrying out necessary investigation procedures to find the source of the OOC result as well as its impact on in-process plant materials.● Use of high-performance liquid chromatography, ultraviolet spectrophotometry, infrared spectrophotometry, colorimetry, and Karl-Fischer automatic titration to determine the concentration of or presence of various impurities in acetaminophen samples.● Performing sampling and testing on all raw materials entering the plant process to ensure their physical and chemical properties meet process standards.● Performing sampling of various water sources used in the chemical processes within the plant weekly.● Performing sampling and testing on wastewater generated by the plant from various sample points in the on-site Activated Sludge Wastewater Treatment plant using HPLC analysis to monitor various pollutant concentrations, and colorimetric analysis to determine chemical oxygen demand and phosphate nutrient concentration.● Keeping up with a fast-paced and dynamic workplace while maintaining attention to detail on a daily basis.● Working on both team-oriented and independent projects.

Henderson, North Carolina, United States

During the duration of my employment at Mako Medical, my responsibilities as a Molecular Technician were:● Proper use of PPE during handling of the SARS-Cov-2 virus including a face shield, double N95 masks, goggles, booties, and a waterproof laboratory coat.● Safe handling and proper storage of live coronavirus nasal swab samples inside and outside of a biosafety cabinet using the Antiseptic Technique.● Preparation of 96 well plates with protein kinase, magnetic bead mix, and an internal MS2 phage control sample in advance of sample loading.● Loading of saline from live SARS-Cov-2 nasal swab samples into prepared 96 well plates from test tubes without cross-contamination of any patient samples.● Extraction of RNA from the SARS-Cov-2 virus using Thermo Scientific’s KingFisher Flex RNA extraction platform after preparing all necessary wash and elution reagents in 96 well plates.● Safe and sanitary handling and storage of RNA extracted from patient samples for later PCR amplification.● Preparation of 384 well plates with reverse transcriptase, Taq-Polymerase, master mix, positive and negative controls, and patient RNA samples for PCR amplification.● Loading and operation of Applied Biosystem’s QuantStudio 12K Flex Real-Time qPCR System.● Interpretation of patient sample qPCR results as positive, negative, or inconclusive in terms of the data acquired from the spectrophotometric analysis of the amplification of the S, N, and ORF1 genes present in the SARS-Cov-2 virus to a specified threshold.● Management of patient information using the Apollo LIMS system.● Maintenance of a clean laboratory environment following procedures developed by Mako Medical Laboratories to meet standards set forth by the College of American Pathologists.

Greensboro/Winston-Salem, North Carolina Area

My work as an undergraduate research assistant under Kimberly S. Petersen included responsibilities such as:•Use of scientific databases such as SciFinder Scholar to find procedures for synthesizing substrates needed for research purposes•Execution of procedures to yield the maximum amount of product possible using an inert atmosphere and flame dried reaction vessel as necessary•Monitoring of reaction progress using thin layer chromatography, gas chromatography, or high-pressure liquid chromatography•Purification of the desired synthesis product using prescribed work up conditions, flash column chromatography, and vacuum conditions for the removal of solvent•Characterization and confirmation of product synthesis through the interpretation of 1H NMR, 13C NMR, 1H COSY, IR Spectroscopy, mass spectrometry, gas chromatography, high-pressure liquid chromatography, and x-ray crystallography as needed•Optimizing computerized methods for the separation of enantiomers using chiral gas and high-pressure liquid chromatography•Storage and handling of reagents and synthesized products according to the conditions mandated by the manufacturer SDS and knowledge of the reactivity of organic functional groups•Creation of laboratory solutions used in wet methods such as the development of thin-layer chromatography plates•Communication and recording of research progress using ChemDraw, Microsoft Powerpoint, Microsoft Excel, Benchling, and written notebooks