Nicholas Corrado Email & Phone Number

Lawrence, Massachusetts, United States

Medford, Massachusetts, United States

Amherst, Massachusetts, United States

Spring 2023Assisted with a study on the effect of different soil compositions on microbial diversity and ecology through production of necromass in soil microcosms - Prepared cellobiose/ammonium-nitrate solution, helped develop formulas to determine the amount of C/N-solution and water to add to keep soils at 45% water holding capacity based off their weight, and weighed and fed all 55 microcosms weeklyPracticed my lab skills - Sterile technique - Pipetting - Making media - Pouring and streaking platesSummer 2023Assisted with building a genome library for a course taught by my PI - Inventoried freezer full of bacterial isolates - Made the media to grow up the desired isolates, performed gDNA extractions on them, quantified the gDNA using a Qubit fluorometer and Nanodrop spectrophotometer, amplified it via PCR, confirmed it was 16S rRNA using agarose gel electrophoresis, sent samples out for sanger sequencing, genotyped samples and cross-checked sequences with archived sequences for the isolates, and performed additional sequencing using a MinION nanopore sequencerMentored high school students performing plasmid isolations - Aided in making reagents, following protocol, and getting accustomed to the labContinued to care for soil microcosms weeklyPrepared to begin my own project in the Fall - Dedicated 4 hours a week to reading and analyzing research papers related to antimicrobial resistance.Fall 2023-Spring 2024Carried out capstone project investigating the effect of long term warming on the spread of antimicrobial resistance in bacteria belonging to the phylum Actinobacteria - Researched, theorized, and crafted literature review around the subject - Optimized MIC assay for environmentally-derived bacteria - Analyzed MIC data using phylognentic comparative methods in R - Wrote a comprehensive thesis and presented a final poster

Amherst, Massachusetts, United States

Worked in the restaurant and served on the In-house, Marketing, Handbook, Hiring, and Bread committees. Attended weekly All-Staff meetings where my fellow co-managers and I discussed issues and gave input to resolve them, as well as strategize better business practices. Established and organized annual live music event in the Sylvan Residential Area.

Medford, Massachusetts, United States

Bachelor Of Science - Bs, Biochemistry And Molecular Biology, Graduate

High School Diploma

Activities and Societies: Marching Band, Key Club