Nicolas Geraud Email & Phone Number

Toulouse, Fr

Toulouse, Occitanie, France

Development of Innovative Catalytic Processes• Enzymatic development and optimization: Design and optimization of enzymatic processes, including catalytic cascades, for sustainable chemical synthesis.• Enzyme engineering and screening: Selection and modification of enzymes through engineering strategies and in silico screening approaches to maximize process efficiency.• Process miniaturization and automation: Development of miniaturized protocols to enhance testing throughput and efficiency.• Biochemical and analytical tools: Development and use of methods for monitoring catalytic cascades and characterizing enzyme performance.• Project management and leadership: Supervision of multidisciplinary teams and management of projects focused on integrated enzymatic solutions.

Toulouse, Occitanie, France

Research Title: Production and Characterization of a New Class of HydrogenasesResponsibilities and Achievements:• Enzyme and Metabolic Engineering: Optimization of biohydrogen production by an engineered bacterial strain.• Molecular Biology Techniques: Application of methods to modify genes and enhance the performance of hydrogenases.• Bacterial Culture and Fermentation: Management of bacterial cultures under anaerobic conditions and conducting fermentation processes (batch, fed-batch, chemostat).• Analyses and Characterizations: Utilization of analytical techniques (HPLC, GC, etc.) to analyze the metabolism of constructed strains.• Enzyme Activity Assays: Conducting in vitro assays under anaerobic conditions to measure the efficiency of hydrogenases.• Production and Purification of Recombinant Proteins: Isolation and purification of produced hydrogenases for in-depth analysis.

Toulouse, Occitanie, France

Research Title: Evaluation of the Predictive Value of Fetal Urinary Inflammatory Markers for Postnatal Renal Function in Fetuses with Posterior Urethral ValvesResponsibilities and Achievements:• Diagnostic Test Development: Participated in the development of a diagnostic test using ELISA and HTRF approaches.• Antibody Screening: Screened an antibody library to establish these diagnostic approaches.• Complex Biological Fluid Analysis: Analyzed complex biological fluids (urine and amniotic fluid) using various techniques (flow cytometry, Simple Western, dot blot, etc.).• Collaboration with a Transfer Company: Collaborated with a technology transfer company for the valorization of the diagnostic test.• Cell Culture: Participated in the culture of primary cells and developed a calcification assay for these cells.

Toulouse, Occitanie, France

Research Title: Development of a Phenotypic Activity Assay for Mycobacterium tuberculosis Protein-O-mannosyltransferase for High-Potential Therapeutic Inhibitor ScreeningResponsibilities and Achievements:• Phenotypic Activity Assay Development: Developed and validated a phenotypic activity assay for high-throughput screening of inhibitors.• Molecular Biology Techniques: Applied molecular biology techniques for constructing enzyme mutants, fusion proteins, and synthetic biology approaches for in vivo enzyme functionalization.• Protein Analysis: Conducted production and purification of recombinant proteins and performed in vitro monitoring of protein-protein interactions.• Microscopy: Used super-resolution microscopy to localize the protein of interest within the bacterium.• Biosafety Protocols: Conducted research in a Biosafety Level 2 laboratory.

Toulouse, Occitanie, France

Teaching practical courses in biochemistry and enzymology

Toulouse, Occitanie, France

Toulouse, France

Specificity of interaction between the HigA antitoxin and the chaperone protein Rv1957 in Mycobacterium tuberculosis.

Doctorat, Biologie Structurale Et Fonctionnelle

Master 2 (M2), Structural And Functional Biochemistry

Master 1, Biotechnology

Licence, Biochemistry And Molecular Biology

Première Année Commune Aux Études De Santé (Paces)

Baccalauréat, Scientifique