Nitya Mittal Email & Phone Number

San Francisco, Ca, Us

South San Francisco, California, Us

- Executes complex cell-based assays and high throughput screens using leading-edge, high-content readouts- Works closely with screening scientists to design assays, run experiments, analyze and interpret results- Collaborates with relevant departments to ensure quality, reproducibility, and relevance of data generation- Conducts cellular screens at scale, using both image-based and transcriptomic readouts, as well as other readouts, as needed.- Troubleshoots and solve problems related to batch effects, imaging protocol selection, cell-line scale up, cell- and compound-dispensing, data acquisition, and instrument scheduling - Works together with the automation teams to develop protocols, carry out optimization experiments with attention to details and careful documentation- Utilizes automated liquid handler systems (i.e., Bravo, Tecan, Hamilton or similar) - Applies strong microscopy skills, including immunohistochemistry, 3D imaging, live cell imaging, and high content imaging- Characterizes mammalian cell cultures via FACS, single cell transcriptomics, or high-content imaging

San Diego, California, Us

- Contributed to experimental design, execution, and data interpretation of projects- Supported discovery pipeline and iPSC platform by following, developing, or troubleshooting protocols for reprogramming of somatic cells into iPSCs, engineering, and/or iPSC cell line generation- Produced human iPSCs using aseptic mammalian cell culture techniques including, media preparation, maintenance of multiple iPSC lines, cell passaging, cell counting, preparing cells for analysis, and cryopreservation- Contributed to iPSC group activities like protocol optimization, cell line derivation and banking, and characterization of generated iPSC lines- Genetically edited/engineered various cell populations, including using CRISPR engineering, and phenotypic and functional characterization related to the genetic modulation- Collaborated and coordinated with cross-functional research and development and teams to advance program objectives- Prepared technical reports and summaries and present data to supervisor, department, or project group as necessary

Cleveland, Oh, Us

Neuronal differentiation of human-induced pluripotent stem cells in vitro - Maintenance of HiPSC cultures by media change, passaging, and single-cell plating - Prepared various recipes for direct differentiation of stem cells using various compounds and cell growth medium manually or using a Tecan liquid handling system (in Biospherix chambers) - Prepared 2D and 3D culture of human-induced pluripotent stem cells, neural progenitors in plates and bioreactors and evaluated the functionality of pluripotent derivatives- Performed immunofluorescence and functional assays to analyze cell differentiation and maturation and also, cryopreservation of pluripotent stem cells- Performed RNA isolation, cDNA synthesis, and qPCR to assess sorted cell populations. - Worked independently with minor supervision with suggestions in modifying, varying, and adapting SOPs in experiments related to culture of the primary neural cells to meet the specific needs of lab research- Analyzed and evaluated experimental data and interpreted results, assessed the importance of findings, prepared graphs and figure sets from primary data- Assisted in the development of new and review of current SOPs and in preparation of presentations and project reports- Operated, calibrated, and performed scheduled maintenance in the laboratory

Kent, Ohio, Us

Study of mitochondrial genetic variation in birds from high altitudes in the Himalayas- Worked on understanding the gene expression changes in local populations of the Tibetan Partridge, Grey Partridge, and Daurian Partridge- Data mined publicly available genome databases to collect published genetic resources on the species of Partridges (Ensembl, NCBI - GenBank, UCSC)- Performed Multiple Sequence Alignment on mitochondrial DNA of the Partridges - Comparative analysis of multiple sequence alignments to identify genetic variants (SNPs and InDels) - Worked proactively with lab members to define analysis questions, interpret analysis results, and prioritize the hypothesis

Kent, Ohio, Us

- Seed, maintain and study cell growth of Liquid Crystal Elastomers (LCEs)- Participate in synthesis and characterization of LCEs under supervision of post-doctorate and graduate students - Prepare porous 3D-LCE foams to create tailored constructs- Demonstrate that both cell guidance realized by LCE morphologies as well as anisometric mechanical properties of the materials are significant for realistic and responsive 3D scaffolds that mimic in vivo microenvironments.- Contribute to literature search for the writing of scientific manuscripts

Provides administrative support to assist the ongoing operations of Kent State University’s research compliance programs. Support Office of Research Compliance staff and investigators in facilitating research that is compliant with federal, state, and local and Kent State University policies.- Correspond with investigators regarding project approvals, revisions, routine questions, and other matters as assigned.- Provide an analytical review of human subjects research applications under the guidance of staff. - Develop reports. - Assist with special projects.- Log new and existing projects and maintain logs.- Perform basic clerical support including filing, preparing meetings, composing letters, invoicing, and other related tasks as assigned.

- Research Analysis- Competitor sheet tracking for 3 months (entering data in master sheet for 5 competitors)- Maintaining 5-7 reports on weekly basis- Intra-team coordination and assisting the management team consisting of 8 people- Work with the production team to ensure client deliverables are met- Understand the digital and publication platform