Numa Piot Email & Phone Number

Saint-Prex, Vaud, Switzerland

Lausanne, Vaud, Switzerland

In Gisou van der Goot laboratory in Life Sciences at EPFL, working on the regulation of anthrax toxin internalisation using two different angles :- Understanding the role of five cytosolic proteins in the process of endocytosis of the two anthrax toxin receptors, CMG2 and TEM8.- Identify actors involved in membrane fusion in late endosomes, process responsible for the release of anthrax toxin in the cytosol, using high-throughput fluorescence microscopy.Main skills acquired :-… Show more In Gisou van der Goot laboratory in Life Sciences at EPFL, working on the regulation of anthrax toxin internalisation using two different angles :- Understanding the role of five cytosolic proteins in the process of endocytosis of the two anthrax toxin receptors, CMG2 and TEM8.- Identify actors involved in membrane fusion in late endosomes, process responsible for the release of anthrax toxin in the cytosol, using high-throughput fluorescence microscopy.Main skills acquired :- Basic biochemistry techniques (Western blot, immunoprecipitation, luminescence assays, etc...)- Molecular biology (multiple cloning strategies, qRT-PCR and analysis)- Mammalian and bacterial cell culture (aseptic technique, human primary cell, plasmid and siRNA transfection, etc...)- Flow cytometry (three colors, intracellular staining)- Fluorescence microscopy (also live-cell)- Good knowledge in Protein purification and Mass spectrometry Show less

Boston, Massachusetts, United States

Master project in Tom Rapoport laboratory studying peroxisomal protein import machinery using the following strategy- Protein expression in yeast cultures- Protein purification using size-exclusion chromatography and affinity chromatography (optimisation)- In vitro protein assays

Master Of Engineering - Meng, Biotechnology

Bachelor Of Engineering - Be, Biotechnology

Maturité, Biology And Chemistry

(In Progress), Applied Data Science : Machine Learning

Certificate Of Completion, Foundations Of Data Science