Patrick Heim Email & Phone Number

Basel, Schweiz

Department: Pharma Technical Development Europe (Biologics)- Analytics (PTDE-A),Section: Bioassay - Automated binding and cell-based potency assays with Hamilton Robotics.- Performed and documented stability and release studies under GMP.- Member of the Bioassay Subteam in the global Automation Task Force. - Lead for global equipment qualification/maintenance and operation documents.- Examined laboratory technicians in biology as elected training expert for canton basel city.Techniques: cell culture, binding assays (ELISA formats), reporter gene/cytokine release/ADCC assays, partial and end-to-end potency assay automation (Hamilton Robotics).Member of the Transformation Team at PTDE (Organizational Development) and Loop Approach Fellow:- Pioneered the path for organizational development, bringing people together and creating full transparency.- Performed workshops to train the Loop Approach for strength- and role-based working within the organization.

Basel

Department: Pharma Technical Development Europe (Biologics)- Analytics (PTDE-A),Section: Bioassay - Automated binding and cell-based potency assays with Hamilton Robotics.- Performed and documented stability and release studies under GMP.- Member of the Bioassay Subteam in the global Automation Task Force. - Lead for global equipment qualification/maintenance and operation documents.- Examined laboratory technicians in biology as elected training expert for canton basel city.- Supervised master student during master's thesis in bioassay automation.Techniques: cell culture, binding assays (ELISA formats), reporter gene/cytokine release/ADCC assays, partial and end-to-end potency assay automation (Hamilton Robotics).Member of the Transformation Team at PTDE (Organizational Development) and Loop Approach Fellow:- Pioneered the path for organizational development, bringing people together and creating full transparency.- Performed workshops to train the Loop Approach for strength- and role-based working within the organization.

Basel, Schweiz

Department: Pharma Technical Development Europe (Biologics) -Analytics (PTDE-A),Section Analytical Development and Mass Spec Analytics- Developed and validated massspectrometric assays for QC of therapeutic proteins.- Performed and documentated release and stability studies under GMP.- Generated reports and sustained a GMP-like laboratory infrastructure.- Analyzed structure-function-relations on therapeutic proteins.- Automated sample preparations with Hamilton Robotics.- Member of QC Sample Prep Automation Global Task Force.- Trained master students and laboratory associates.- Organized an international 2day department summit for appr. 200 employees.Techniques: MALDI-TOF (Bruker Daltonics), LC/MS (Waters/Bruker Daltonics), UPLC/HPLC (Waters/Agilent), protein chemistry, lab automation (Hamilton).

Basel, Schweiz

Department: Pharma Technical Development Europe (Biologics) -Analytics (PTDE-A),Section Analytical Development and Mass Spec Analytics- Developed and validated massspectrometric assays for QC of therapeutic proteins.- Performed and documentated release and stability studies under GMP.- Generated reports and sustained a GMP-like laboratory infrastructure.- Analyzed structure-function-relations on therapeutic proteins.- Automated sample preparations with Hamilton Robotics.- Member of QC Sample Prep Automation Global Task Force.- Trained master students and laboratory associates.- Organized an international 2day department summit for appr. 200 employees.Techniques: MALDI-TOF (Bruker Daltonics), LC/MS (Waters/Bruker Daltonics), UPLC/HPLC (Waters/Agilent), protein chemistry, lab automation (Hamilton).

Sigmaringen, Baden-Württemberg, Deutschland

Holding lectures in the study curse Bioanalytic (6th semester):- Presented about mass spectrometric assays for QC of therapeutic proteins in the module Bioassay 2. - Presented about the automation of potency assays in the module Lab Automation 2.

Basel

Department Drug Metabolism and Pharmacokinetics (DMPK)- Cultivated primary hepatocytes and performed drug metabolism studies.- Developed RP-HPLC methods for the detection of drug metabolites.- Cultivated organotypic cell culture and performed biochemical assays.- Designed models for microfluidic flow rate.- Masterthesis: Evaluation of a primary hepatocyte longterm culture system CELLASIC.Techniques: Cell proliferation assays, lactate dehydrogenase detection, CYP 3A4 activity assay, BCA protein determination, microscopy, cell culture handling, microfluidics, reversed phase chromatography (Shimadzu).

Penzberg

Department: Reagent Development and Rare Reagents- Evaluated biomarkers (autoantibodies) for early detection of cancer by human sera screening .Techniques: Size exclusion chromatography (Agilent), enzyme linked immunosorbent assays, human sera handling, protein coating/immobilization/conjugation, protein chemistry.

Penzberg

Department: Reagent Development and Rare Reagents - Performed protein folding in vitro (chaperone fusion proteins/rapid dilution approach).- Evaluated biomarkers (sensitivity/specifity) by clinical sample screening.- Developed and optimized immunological reactants and buffer compositions for ELISAs.- Project work: Significance of protein folding for the development of ELISAs.- Bachelor thesis: Development of an indirect ELISA for the detection of auto antibodies against tumor-associated antigens.Assays: protein folding (rapid dilution), dialysis, size exclusion chromatography (Agilent), UV protein determination, enzyme linked immunosorbent assays, human sera handling, protein coating/immobilization/conjugation, protein chemistry, SDS Page, Westernblot.

Mannheim

Department: Decentralized Solutions and Reagent Development- Evaluated drug-biomarker interactions on Roche's cobas h232 and Cardiac reader.- Developed ELISAs for acute myocard infarct detection (cardiovascular biomarkers).- Evaluated a novel microfluidic lab-on-chip system for point of care testing.- Internship report: Immunoassay optimization for the detection of cardiovascular biomarkers.Assays: ELISAs with fluorophor-conjugated antibodies, fluorescent microscopy, human sera handling/screening, hematocrit detection, microfluidic ELISAs, protein coating/immobilization.

Master Of Science (M. Sc.), Biomedical Sciences

Activities and Societies: Allgemeiner Studentenausschuss (ASTA). Elected student member in university senate. Tutor in mathematics.

Bachelor Of Science (B. Sc.), Pharmatechnik (Bio Engineering)

Activities and Societies: Allgemeiner Studentenausschuss (ASTA). Tutor in mathematics and physics. Supervision of celebrations. University.