Global category manager in bioprocess materials for filtration and disposables

Global category manager in bioprocess materials for filtration and disposables

-Extensive experience in filtration and clarification including precipitant and flocculation technologies.-Development engineer for the Clarisolve family of clarification devices. Designed, executed and analyzed dozens experiments for best practices documents and recommendations, for internal and external facing documents.-Hosted demonstrational video for the internal training of process development scientists.-Developed sample preparation method for cationic polymer quantification utilizing qPCR.-Applications development work to understand various polymer's dosing and residual effects on downstream protein purification including toxicity, resin cycling studies, impurity removal and product yield across Clarification, Sterile Filtration, Protein-A, CEX and AEX operations.-Numerous customer trials for process development/implementation studies domestically and internationally.-Developed and authored several SOP's for manufacturing, best practices and experimental protocols.-Extensive analytical equipment experience, FRBM, HPLC, Akta Explorer, Pendotech NFFSS, qPCR.-Presented poster at the 2012 BioProcess International conference in Providence Rhode Island.-Generated, analyzed and presented data for model development for the scaling of chromatography resin in packed bed columns-Generated, analyzed and presented data to understand static binding capacity and ligand density of surface modified chromatographic resin.

Followed SOP's for media preparation, cellular cultivation, storage, and analysis of cell culture Kept detailed Excel files of raw and analyzed data, in addition to a summary of experiment procedures, parameters, and methods Worked in a team cGMP environment to thaw, grow, and maintain mammalian cell cultures using sterile techniques in shake flasks, wave bags, and bioreactors Designed experiments using chemically defined and hydrolysate media with various amino acid, vitamin, metal, and other additives to optimize cell growth, product quality, titer, and other cell growth characteristics of interest Maintained a detailed lab notebook following cGMP methods containing detailed laboratory observations, reproducible method explanation, raw data entry, and quantified analytical data for method improvement recommendations Prepared, Inoculated, Operated, and Harvested Applikon Bioreactors. Daily tasks included data logging, correcting operating parameters, and maintained cell growth requirements and feeding cycles Quantified various cellular operating parameters using a YSI 7100, an Innovatis GmbH Cedex Cell Counter, a Precision Systems Osmette Micro Osmometer, an ABL800 BGA, along with various other bio-analytical laboratory equipment Responsible for daily lab upkeep and maintenance of analytical equipment and sensors

Designed, operated, and maintained small scale pilot to simulate hot water heaters to study disinfection byproduct kinetics Worked with peers for analytical sample extraction for GC, IC, HPLC/MS, TOC, and TOX analysis to study formation of various disinfection byproducts, using EPA and in-house standard operating procedures (SOP), while providing useful feedback for method improvements Analyzed and interpreted GC, IC, UV-Vis spectrophotometer, and TOC results and presented results in Excel and SigmaPlot to peers and professors Implemented and improved and operated three water filtration systems to completion in order to study their effectiveness over several months Designed, made equipment purchase recommendations, and implemented transportation and storage system to transport 3000-lbs of water from sampling site to laboratory to improve sampling efficiency and reduce possible injuries which was completed 28% under budget

• As part of a pilot scale test, used ceramic microfiltration membranes to remove solid char particles from fast pyrolysis bio-oils to improve stability and combustion properties• Improved separation system design to reduce cleaning chemical consumption by more than 50% , reduce down time by more than 25%, and reduce product waste through, handling methods and recycle feeds• Used optical microscopy to quantify char particle removal from filtered Biodiesel on a micron scale• Conducted permeation studies and did data recording and analysis for later use in research papers