Phil Colgan Email & Phone Number

Durham, North Carolina, United States

Lead the Computational and Automated Biology team.Develop high throughput automated workflows using multi-peripheral robotic platforms including Hamilton Vantage / Venus and PAA / Overlord platforms. Workflows developed include 2 stage microfermetation, biobanking, colony isolation.Implement and maintain infrastructure for standardized automated data collection and analyses via R and Python scripting as well as API integrations with LabGuru ELN.

Develop, validate and stand-up multi-instrument, high-throughput automated platforms including HTP phage engineering, HTP NGS (gDNA extraction, quantification, library prep, MiSeq), HTP biobanking, HTP qPCRWrite scripts and analysis pipelines in version-controlled, cloud-based computing environments to integrate multi-instrument automated workflows with emphasis on collecting robust, traceable dataOperate, maintain, troubleshoot, and communicate with service engineers to maximize production time for the automated equipment

Ames, Ia

Engineered bacterial reporter strains with recombinant plasmids and developed an associated conjugation assay resulting in a novel method for detecting actively mobile gene cassettes in co-cultureOptimized methods for a Biomark HD microfluidic HT-qPCR platform resulting in an assay allowing simultaneous quantification of 44 antibiotic resistance and mobility genes in unamplified DNA from complex soil, water, and manure samplesPresented modules and provided teaching assistance at 3 national and international workshops for metagenomic analysis and handling big data using Linux Bash Shell, Python, Microsoft Excel, and R incuding phyloseq, vegan, DeSeq2, and ggplot2 packages

East Lansing, Michigan

Designed and synthesized species-specific nanoparticle-functionalized DNA probes resulting in 2 novel nanoparticle-based DNA biosensor assays for detection of 14 bacterial, fungal, and viral plant pathogens

East Lansing, Mi

Isolated pure cultures of over 200 bacteria and fungi from the rhizosphere soil of switchgrass and screened for plant growth promoting functional genes using amplicon sequencing followed by functional verification with RT-qPCR and wetlab assays

Lansing, Michigan Area

Prepared reagents for, assembled, and supervised the general, forensic and organic chemistry laboratories.

Greater Grand Rapids, Michigan Area

Machined and welded hydraulic and pneumatic cylinders utilizing MIG, TIG, Flux core, SMAW, and oxy-acetylene welding as well as plasma, oxy-acetylene, and arc-gouge cutters in all positions and on hot and cold rolled steel, aluminum and titanium.Programmed and operated CNC mills and presses.

Eaton Rapids, Michigan

Designed and built custom 3rd and 4th-axis CNC machines per customer specifications including 3.2-6 kW ESAB laser cutting systems, plasma cutting systems, water-jet cutting systems, ultrasonic and tangential drag knife systems, digitizing probe and laser digitizing 3-D scanners.Traveled for on-site technical support, set-up and training

Phd, Agricultural And Biosystems Engineering

Bachelor'S Degree, Genomics And Molecular Genetics