Phuong Huynh Email & Phone Number

Morrisville

Technical Data Reviewer

Research Triangle Park, Nc

Responsible for conducting biotransformation studies with an emphasis on the ability to identifiy metabolites by mass spectrometry•Produce robust UPLC/HPLC-MS/MS chromatographic methods for the separation, quantification, isolation, purification, and identification of drug metabolites in various biological matrices in support of preclinical development•Responsible for the quantitative and qualitative analysis (radiochemical detector, API-5000, Q-trap, LTQ, Orbi-trap, and Synapt G2-S) of drugs and metabolites in various biological fluids to structurally identify metabolites•Study director/study monitor for in vivo preclinical PK/mass balance studies•Conduct metabolism studies using in vitro systems (microsomes, hepatocytes and S9) •Develop and perform liquid to liquid extraction, solid phase extraction, protein precipitations and derivative techniques in the extraction of analytes from biological fluids and tissues•Accountable for the operation of LC/MS, radiochemical detector, liquid scintillation counter and microplate scintillation and luminescence counter to generate on-line/off-line metabolic profiles•Generate data and reports suitable for regulatory filings in compliance with SOPs and relevant GLP requirements

Groton, Ct

•Developed and applied LC/MS/MS methods to quantitate samples of potential drug candidates•Calculated the pharmacokinetic parameters of preclinical drug candidates using in vivo (mouse, rat, dog and monkey) and in vitro (liver microsomes, cytosols, hepatocytes and protein binding) systems•Designed experimental protocols to conduct protein binding in plasma/serum, microsomes and brain tissue to assess and predict the fraction unbound in vitro versus in vivo in both discovery and development settings (Ultrafiltration, 96-wells format and Equilibrium Dialysis protein binding methods)•Conducted various in vivo dosing (oral, intravenous and intramuscular) and sampling (blood, plasma, serum, cerebrospinal fluid and organs) techniques, including the use of the AccuSampler ABS, an automated blood sampling unit•Designed, executed, analyzed, documented and reported results of pharmacokinetic and metabolism studies in rodent, canine and non-human primate to characterize the disposition and metabolism of potential drug candidates•Conducted blood to plasma stability and red blood cell partitioning studies•Knowledge and experiences in working safely with radioisotopes and biological materials