Patrick Niekamp Email & Phone Number

Prepared, reliable, and goal-oriented scientist with nine years of research experience in cancer immunology, molecular biology, and bioinformatics. Technical skills:• NGS applications, such as scRNA-seq, scTCR-seq, and ChIP-seq• Generation, in vitro validation, and in vivo testing of CAR T cells• Pharmacokinetic and pharmacodynamic studies• Viral library preparation, virus purifications, and transductions• In vivo experimental design and execution, including T cell and cancer cell injections• Molecular cloning, DNA and RNA isolation, RT-qPCR, and side-directed mutagenesis • Protein expression, purification, and testing using electrophoretic techniques and immunoassays• Isolation of immune cells from various organs and tissues, such as blood, tumors, and bone marrow• Cultering and differentiation of iPSCs• Generation of knock-out and knock-in cell lines using the CRISPR/Cas9 system • Flow cytometry, immunohistochemistry, and fluorescence microscopyData Analysis• R for advanced data analysis, statistics, and NGS analysis• Experienced in using GitHub for version control, collaboration, and code review • Experience using SQL databases• Python programming • GraphPad Prism• Power BIHobbies:• Table tennis, squash, and tennis• Kickboxing and MMA • ChessFavorite books:• Non-fiction: The Emperor of All Maladies - Siddhartha Mukherjee• Fiction: Malazan Book of the Fallen Series - Steven Erikson & Ian Cameron Esslemont