Purnima Singh Email and Phone Number
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With over 5 years of research and work experience in molecular biology and bioprocessing, I currently work at Catalent Pharma Solutions (CDMO) in the Downstream Processing - Manufacturing Sciences and Technology group (MS&T). During my second master's at Keck Graduate Institute, I was Research Assistant for two projects; one where I focus on the assembly of light chain and heavy chain in E.coli and P.pastoris for generation of antibody library against SARS COVID antibody. Two, planning, organizing and running 50L bioreactor (scaling up of 50 million CHO cells with more than 93% viability) in fed-batch mode for Repligen Co-operation. My goal is to contribute to the development of novel biopharmaceutical products and processes that can improve human health and well-being.Previously, I worked as a Process Engineer and Researcher at KGI, Repligen Corporation and Boehringer Ingelheim, where I gained valuable experience in scaling up CHO cell culture in a 50L Bioreactor (Upstream Processing), purifying monoclonal antibodies, and optimizing protein purification methods specifically for Affinity Chromatography (Downstream Processing). I also have a strong background in plant and animal tissue culture along with algal transformation, CHO, and stem cell process development, which I acquired through my academic and industrial projects.
Catalent Pharma Solutions
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Process Engineer-Ms And T DownstreamCatalent Pharma SolutionsClaremont, Ca, Us
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Process Engineer-Ms&T DownstreamCatalent Pharma Solutions Apr 2024 - PresentMadison, Wisconsin, United States
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Research AssistantKeck Graduate Institute Feb 2022 - Apr 2024Claremont, California, United StatesIdentification of fungal strains using qPCRIsolation of strains and transformation in E.coliConstruction of SARS Covid Antibody Library; screening of Light Chain (LC) and Heavy Chain (HC) Antibodies, glycerol stock preparation and mini-prep of LC and HCLinearization of vectors before cloning, Preparation of plates and media for transformation -
Research AssistantRepligen Corporation Oct 2022 - Jun 2023Claremont, California, United StatesScaled up the culture from 20 ml in a shake flask to 50L bioreactor, making CHO cell bank, media preparation, planning and execution of the project, leading the team of three people.Running 50L Bioreactor in fed-batch mode with 22 million VCD at 93% viabilityMonitoring metabolite production and gas levels -
Technology Innovation InternBoehringer Ingelheim May 2022 - Aug 2022Fremont, California, United StatesMonoclonal Antibody Purification: using AKTA and Unicorn Software1) Evaluation of Fibro PrismA device with Mabselect PrismA in flow-through mode2) Evaluation of Workbeads 40 TREN as multimodal IEX on Fibro PrismA (lifetime study) and Mabselect PrismA3) Collected several eluate fractions for analysis of high and low molecular variants using SEC-HPLC4) Analysed the presence of Host Cell Protein using the HCP-Gyros quantification method5) Learned the art of Column Packing6) Used Bio-HT for Antibody titer Analysis and SoloVPE/Tecan for protein quantification7) Optimized the Method to increase the protein purification efficiency on Unicorn Software8) Performed simulation using DSPX software-Gosilico Chromatography for Mechanistic Modelling -
Graduate Student ConsultantSanbio, Inc. Oct 2021 - May 2022Keck Graduate InstituteFall Project focused on process development of hBM-MSCs from T-75 flask to CS-10: Cell growth kinetic study on Human Mesenchymal Stem Cells (hMSCs) before and after transfecting with the therapeutic product.Spring Project focused on Analytical Development: To perform different MSC Licensing studies using High Throughput methods such as BLITz (Octet), Exosome Induction, Isolation, and purification, followed by quantification, analysis, and it's characterization.Led the team of three on exosomes induction, isolation, purification and it's characterization -
Project ManagerMeera Cleanfuels Limited Jun 2021 - Aug 2021Mumbai, Maharashtra, IndiaDelegated work on starting labs and mentoring 3 employees for completion of projectConnecting with vendors and professors on execution of projects -
Senior Project EngineerMeera Cleanfuels Limited Feb 2019 - May 2021• Delegated the team of Biofuel project “Microbial consortium and process parameters for Biogas production” in collaboration with Institute of Chemical Technology-Mumbai and Indian Institute of Technology-Bombay • Planned and mediated the cultivation of optimum biomass and optimization of the microbial consortium with process parameters for increased biogas yield. Prepared documents for submission of proposals for government projects•Arranged materials for lab set-up, designed protocols, Lab and bioreactor bill of quantities (BOQ). Interviewed, mentored and trained new interns on the Bioprocess project. Recruited and coordinated with consultants for outsourcing the work towards completion of project
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Research InternReliance Industries Limited Jan 2018 - Jul 2018Navi Mumbai, Maharashtra, India• Won Scholarship from BCIL-BITP, Department of Biotechnology (DBT), Government of India to work on the topic “Transgene expression, Optimization and Characterization of reporter genes in Chlamydomonas reinhardtii CC125”• Transformation in algal expression system using pChlamy_4: Gene X vector in Chlamydomonas• Optimization of Transformation efficiency in Chlamydomonas using two different reporter genes• Designing and Standardization of different PCR primer sets for two different gene of interest• Performed Molecular cloning of gene of interest in a bacterial system and algal system for laying the proof of concept with successive results, maintenance of Axenic culture with scale-up for standardizing the optimum media -
Junior Safety Data AnalystSciformix Dec 2016 - Dec 2017Mumbai Area, IndiaWorked on Johnsons and Johnsons - MDC Project (Pharmacovigilance)• Executed case processing activities, including ICSRs intake, triage of cases (EMEA, APAC, LATAM, and the US) based on seriousness criteria, performed duplicate searches, data entry of individual case reports, MedDRA coding, causality, and writing case narratives in the RSS safety database for documentation of reports• Performed follow up, query management, was involved in the weekly meetings with consumer contact center, managed outbound and inbound letters, collaborated with the medical and quality team for maintaining the efficacy of the reports -
Dissertation TraineeAdvanced Centre For Treatment, Research And Education In Cancer(Actrec) Dec 2015 - May 2016Kharghar, Navi MumbaiEpigenetic and Chromatin Biology Lab: Sanjay Gupta Lab, Cancer Research Institute, ACTREC-TMCThesis Title: “Generation and epigenetic characterization of radioresistant MDA-MB231 breast cancer cell line”• Generation of radioresistant MDA-MB 231 cell line by fractionated ionizing radiation method, 2Gy-12Gy• To study the changes in histone post-translational modifications acquired during the generation of radio-resistant MDA-MB 231 cell line• Changes in cell migration & chromatin architecture between normal and 12Gy radioresistant cell line -
Internship TraineeNational Burn Centre Jun 2015 - Jul 2015Airoli, Navi Mumbai, India• Training in Skin Banking in the Skin Regeneration lab for two weeks at National Burn Center in Jul 2015
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Project InternHaffkine Institute For Training, Research & Testing Dec 2013 - Apr 2014Mumbai Area, Indiao Plant Tissue Culture Department for a month which extended further by several months from Dec 2013 to Apr 2014, project funded by Department of Science and Technology (DST), Government of India1. Production of secondary metabolites like kaempherol using antioxidants like jasmonoic acid in Tylophora Indica in Zenk production media.2. Production of secondary metabolites like curcuma using antioxidants like jasmonoic acid in Curcumin longa in Zenk production media.3. Effect of calendar on production of phenols in Tylophora Indica and Curcumin longa. o Attended Annual Biotechnology Training Program for two weeks in Jun 2013o Attended one day workshop on Mission to control mosquito menace in Jul 2013
Purnima Singh Skills
Purnima Singh Education Details
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Biopharmaceutical Processing
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School Of Biotechnology And Bioinformatics, D.Y.Patil UniversityBiotechnology -
Central Academy Senior Secondary SchoolScience
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Adani D.A.V Public School9Th-10Th Standard
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D.A.V Public School7Th-9Th Standard
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Atomic Energy Central School3Rd-6Th Standard
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Mount Carmel High School1St-2Nd Standard
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Greenhill Academy High SchoolSr.Kg
Frequently Asked Questions about Purnima Singh
What company does Purnima Singh work for?
Purnima Singh works for Catalent Pharma Solutions
What is Purnima Singh's role at the current company?
Purnima Singh's current role is Process Engineer-MS and T Downstream.
What is Purnima Singh's email address?
Purnima Singh's email address is pu****@****mix.com
What schools did Purnima Singh attend?
Purnima Singh attended Keck Graduate Institute, School Of Biotechnology And Bioinformatics, D.y.patil University, Central Academy Senior Secondary School, Adani D.a.v Public School, D.a.v Public School, Atomic Energy Central School, Mount Carmel High School, Greenhill Academy High School.
What are some of Purnima Singh's interests?
Purnima Singh has interest in Social Services.
What skills is Purnima Singh known for?
Purnima Singh has skills like Molecular Biology, Microsoft Office, Management, Microsoft Word, Polymerase Chain Reaction, Powerpoint, Research, Microsoft Excel, Biotechnology, English, Market Research, Public Speaking.
Who are Purnima Singh's colleagues?
Purnima Singh's colleagues are Ely Peng, Maryam Moghtaderi, Kassandra Ariza, Mary Foster, Elizabeth Castle, Tyrone Ferrens, Jose Acosta.
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Purnima Singh
Clinical Research Laboratory Manager | Associate Professor | Biorepository Operations Expert | Genomics, Epigenomics And Cancer Genetics Researcher | Lead Scientific Innovation And Interdisciplinary CollaborationGreater Birmingham, Alabama Area1uab.edu -
2addiox.com, flextoninc.com -
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