Purnima Singh Email & Phone Number

Claremont, Ca, Us

Madison, Wisconsin, United States

Claremont, California, United States

Identification of fungal strains using qPCRIsolation of strains and transformation in E.coliConstruction of SARS Covid Antibody Library; screening of Light Chain (LC) and Heavy Chain (HC) Antibodies, glycerol stock preparation and mini-prep of LC and HCLinearization of vectors before cloning, Preparation of plates and media for transformation

Claremont, California, United States

Scaled up the culture from 20 ml in a shake flask to 50L bioreactor, making CHO cell bank, media preparation, planning and execution of the project, leading the team of three people.Running 50L Bioreactor in fed-batch mode with 22 million VCD at 93% viabilityMonitoring metabolite production and gas levels

Fremont, California, United States

Monoclonal Antibody Purification: using AKTA and Unicorn Software1) Evaluation of Fibro PrismA device with Mabselect PrismA in flow-through mode2) Evaluation of Workbeads 40 TREN as multimodal IEX on Fibro PrismA (lifetime study) and Mabselect PrismA3) Collected several eluate fractions for analysis of high and low molecular variants using SEC-HPLC4) Analysed the presence of Host Cell Protein using the HCP-Gyros quantification method5) Learned the art of Column Packing6) Used Bio-HT for Antibody titer Analysis and SoloVPE/Tecan for protein quantification7) Optimized the Method to increase the protein purification efficiency on Unicorn Software8) Performed simulation using DSPX software-Gosilico Chromatography for Mechanistic Modelling

Keck Graduate Institute

Fall Project focused on process development of hBM-MSCs from T-75 flask to CS-10: Cell growth kinetic study on Human Mesenchymal Stem Cells (hMSCs) before and after transfecting with the therapeutic product.Spring Project focused on Analytical Development: To perform different MSC Licensing studies using High Throughput methods such as BLITz (Octet), Exosome Induction, Isolation, and purification, followed by quantification, analysis, and it's characterization.Led the team of three on exosomes induction, isolation, purification and it's characterization

Mumbai, Maharashtra, India

Delegated work on starting labs and mentoring 3 employees for completion of projectConnecting with vendors and professors on execution of projects

• Delegated the team of Biofuel project “Microbial consortium and process parameters for Biogas production” in collaboration with Institute of Chemical Technology-Mumbai and Indian Institute of Technology-Bombay • Planned and mediated the cultivation of optimum biomass and optimization of the microbial consortium with process parameters for increased biogas yield. Prepared documents for submission of proposals for government projects•Arranged materials for lab set-up, designed protocols, Lab and bioreactor bill of quantities (BOQ). Interviewed, mentored and trained new interns on the Bioprocess project. Recruited and coordinated with consultants for outsourcing the work towards completion of project

Navi Mumbai, Maharashtra, India

• Won Scholarship from BCIL-BITP, Department of Biotechnology (DBT), Government of India to work on the topic “Transgene expression, Optimization and Characterization of reporter genes in Chlamydomonas reinhardtii CC125”• Transformation in algal expression system using pChlamy_4: Gene X vector in Chlamydomonas• Optimization of Transformation efficiency in Chlamydomonas using two different reporter genes• Designing and Standardization of different PCR primer sets for two different gene of interest• Performed Molecular cloning of gene of interest in a bacterial system and algal system for laying the proof of concept with successive results, maintenance of Axenic culture with scale-up for standardizing the optimum media

Mumbai Area, India

Worked on Johnsons and Johnsons - MDC Project (Pharmacovigilance)• Executed case processing activities, including ICSRs intake, triage of cases (EMEA, APAC, LATAM, and the US) based on seriousness criteria, performed duplicate searches, data entry of individual case reports, MedDRA coding, causality, and writing case narratives in the RSS safety database for documentation of reports• Performed follow up, query management, was involved in the weekly meetings with consumer contact center, managed outbound and inbound letters, collaborated with the medical and quality team for maintaining the efficacy of the reports

Kharghar, Navi Mumbai

Epigenetic and Chromatin Biology Lab: Sanjay Gupta Lab, Cancer Research Institute, ACTREC-TMCThesis Title: “Generation and epigenetic characterization of radioresistant MDA-MB231 breast cancer cell line”• Generation of radioresistant MDA-MB 231 cell line by fractionated ionizing radiation method, 2Gy-12Gy• To study the changes in histone post-translational modifications acquired during the generation of radio-resistant MDA-MB 231 cell line• Changes in cell migration & chromatin architecture between normal and 12Gy radioresistant cell line

Airoli, Navi Mumbai, India

• Training in Skin Banking in the Skin Regeneration lab for two weeks at National Burn Center in Jul 2015

Mumbai Area, India

o Plant Tissue Culture Department for a month which extended further by several months from Dec 2013 to Apr 2014, project funded by Department of Science and Technology (DST), Government of India1. Production of secondary metabolites like kaempherol using antioxidants like jasmonoic acid in Tylophora Indica in Zenk production media.2. Production of secondary metabolites like curcuma using antioxidants like jasmonoic acid in Curcumin longa in Zenk production media.3. Effect of calendar on production of phenols in Tylophora Indica and Curcumin longa. o Attended Annual Biotechnology Training Program for two weeks in Jun 2013o Attended one day workshop on Mission to control mosquito menace in Jul 2013

Master'S Degree, Biopharmaceutical Processing

KGI MEng Presidential Scholarship Fenton Bioprocessing Leadership Award Projects: 1) Upstream Bioprocessing: Worked on producing CHO-S.

M.Tech Integrated, Biotechnology

11Th-12Th, Science

9Th-10Th Standard

7Th-9Th Standard

3Rd-6Th Standard

1St-2Nd Standard

Sr.Kg