Puspendra Singh Email and Phone Number

• Developed and implement semi high throughput phenotyping assay related to drug resistance evolution in malaria parasite.• Dissecting the genetic complexity of artemisinin resistance in P. falciparum Malaria.• Phenotyping and genotyping of malaria parasite.

• Developed a tissue culture facility and worked on mesenchymal stem cell differentiation in 2D versus 3D environment.• Maintenance of a mammalian tissue culture facility performed and supervised experiments related to the development of tissue constructs, and the treatment of diseased tissue, such as cancer.• Developed the treatment vehicles such as those based on hydrogels and nanoparticles, performed gene expression studies, and analyzed of the resulting data.

• Worked on the Identification of virulent genes responsible for cerebral and severe malaria and delineate the variations between these two forms of malaria.• Assessed the efficacy and safety of the combination Artemether-Lumefantrine for the treatment of uncomplicated Plasmodium falciparum malaria infection.• Studied the "HRP2 and HRP3 expression in Plasmodium falciparum parasites to identify next-generation mutation from the endemic state of India: A prospective evaluation.

ICMR Fellow on "Immunological Responses associated with pathogenesis & protection against Malaria during Pregnancy".

 Prepared a field site for malaria vaccine trial in & around Jabalpur (M.P.), Site Census and supervise all the field work & Lab Work. Found pf-CSP protein genetic variation in India and its relevance to RTS S vaccine of malaria. Profiling of pro- and anti-inflammatory cytokine in Plasmodium vivax malaria patients from central zone of India Evaluated a rapid diagnostic test “First Response Malaria Combo (pLDH/HRP2)” card test, for malaria diagnosis in the forested belt of… Show more  Prepared a field site for malaria vaccine trial in & around Jabalpur (M.P.), Site Census and supervise all the field work & Lab Work. Found pf-CSP protein genetic variation in India and its relevance to RTS S vaccine of malaria. Profiling of pro- and anti-inflammatory cytokine in Plasmodium vivax malaria patients from central zone of India Evaluated a rapid diagnostic test “First Response Malaria Combo (pLDH/HRP2)” card test, for malaria diagnosis in the forested belt of Central India. Show less

 Developed the specific microbial packages for the treatment of paper & pulp industrial waste effluent. Isolate & Screened the bacteria (Alkalophilic) for neutralizing alkaline waste effluent of beverage industries from soil. Biological Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) analysis of waste water.