Yi Quan Email & Phone Number

San Francisco Bay Area

Curriculum & Instruction

Menlo Park, Ca

Los Altos Hills, Ca

Communication Studies and Child Development

Belmont, Ca

Lead high throughput discovery platform for isolating ultra-rare therapeutic-grade antibodies directly from the blood of humans• Culture PBMC from cancer patients• High throughput sequencing single B cell• Monoclonal antibody discovery and engineering

Manage the lab; oversee supply & equipment budget; work with health & safety department; participate in multidisciplinary teams across different labs• Coordinate study and collaborate with 10+ scientists/grad students on interactions of electric field and light with biological cells (Rodent Primary Neuronal Culture with spontaneous spikes) and tissues (Rat or Rabbit Retina) using Microelectrode Array (MEA), Fluorescent Angiography and Immunohistochemistry• Built relationships with 5+ lab supply vendors negotiating advantageous prices and deliveries• Train new lab staff on all health and safety regulations ensuring health & safety compliance

Greater New York City Area

Conducted genome editing of LRRK2 gene in human induced pluripotent stem cells using engineered transcription activator-like effector nucleases• Used different cloning techniques (Traditional cloning and TOPO sub-cloning) to make homozygous mutant PCR fragments from human brain genomic DNA with heterozygous mutation• Knocked-in LRRK2 gene mutation to iPSC culture by electroporation using TALEN and homologous recombination techniques; colony screening, expansion and characterization

Gainesville, Florida Area

Conducted independent research to explore the therapeutic effect of carbon monoxide(CO) on primary cortical neuronal death caused by oxygen-glucose deprivation(OGD)• Established protocols to get primary neuronal culture to study ischemic stroke• Discovered that CO can prevent neuronal death caused by OGD

Studied COX-2 signaling in epilepsy and immunomodulation of microglial activation by EP2 receptors• Focused on the development and characterization of EP2 Receptor Antagonist using a cell-based homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) method measuring cytosolic cAMP in C6 glioma (C6G) cells overexpressing human EP2 receptor• Evaluated 10+ inflammation markers in primary cultured microglia with or without classical activation by real-time PCR, ELISA and Western Blot• Identified the EP2 receptor signaling pathways regulating microglial activation

Investigated the Fc receptor-mediated phagocytosis in microglia, flow cytometry and Immunocytochemistry were used to evaluate phagocytosis of fluorescein labeled Staphylococcus aureus• Studied the regulation of Fcγ receptors and immunoglobulin G-mediated phagocytosis in mouse microglia• Demonstrated that IgM-dependent phagocytosis in microglia Is mediated by complement receptor 3, not Fcα/μ receptor