Rahul Bhattacharjee, Phd Email & Phone Number

Boston, Ma, Us

Boston, Ma, Us

South San Francisco, California, Us

Nashville, Tennessee, Us

• Developed/established new methods/protocols de novo as required.• Reviewed and critically interpreted published work to guide internal research.• Designed and executed complex in vivo and in vitro experiments in Scizosaccharomyoces pombe and collaboration with a multi-disciplinary project team, including computational biophysicists to elucidate the role of F-BAR protein Cdc15 in regulation of cytokinesis.• Leading efforts to identify the role of intrinsic disordered region (IDR) of Cdc15 in driving liquid-liquid phase separation to form membrane bound condensates.• Routinely developed and ran simple one step and complex programs in AKTA pure (Cytiva) system to purify recombinant proteins from bacteria using affinity chromatography and size exclusion chromatography column.• Regularly performed quantitative biochemical binding assays with recombinant proteins and synthetic peptides using Isothermal Titration Calorimetry.• Experience in working with radioactive isotope (32P) to perform simple and complex kinase and phosphatase assays.• Performed TIRF microscopy and wide field microscopy to show role of the IDR containing F-BAR protein Cdc15 driven phase separation during cytokinesis. Performed in-vitro liquid-liquid phase separation assays to finally analyze them under TIRF microscopy and wide field microscopy.• Collaborated and effectively communicated with computational biophysicists to analyze the role of the IDR region of F-BAR protein Cdc15 in phase separation in a phosphorylation dependent manner.• Regularly performed genetic analysis using different mutant strain of Scizosaccharomyoces Pombe.• Performed biochemical assays using lipids regularly. Experienced in making both Small Unilamellar vesicles (SUVs) and Giant Unilamellar Vesicles (GUVs) to perform binding assay experiments.• Wrote original manuscripts. Collaborated with scientists (within and outside lab) resulting in 4 co-authored publications.

Kent, Ohio, Us

Elucidated that Glycogen Synthase Kinase-3a has isoform specific role in male fertility.• Crossed mice to develop novel Cre-lox model, used this for ex vivo and in vivo studies to study role of Glycogen Synthase Kinase-3a in spermatogenesis and male fertility.• Experienced in isolating DNA from mouse ear and tail to perform genotype identifications.• Performed ex-vivo biochemical analysis with mouse spermatozoa for different types of biochemical assays (immunoprecipitation and pull-down experiments), sperm culture, cellular assays, qRT-PCR, western blots, confocal microscopy, and live cell imaging.• Regularly performed in-vivo and in-vitro biochemical assays involving mouse spermatozoa isolated from wild-type and KO mouse and recombinant proteins made from bacteria.• Experience in working with radioactive isotope (32P) to perform simple and complex kinase and phosphatase assays.• Established co-staining Immunofluorescence (IF) and IHC protocols to study proteins involved in spermatogenesis form mouse and bull spermatozoa and paraffin embedded mouse tissue samples.• Collaborated with bioinformaticians and MS core to analyze LCMS-MS data on samples isolated from WT and KO mouse spermatozoa.• Wrote manuscripts, IACUC protocols, RO1 and other format grants/fellowships.• Collaborated with scientists (within and outside lab) resulting in 4 co-authored publications.• Invested in training and mentoring of undergraduates and rotation students in laboratory.• Directly supervised junior rotation and undergraduate students in the lab.• Excellent ability to thrive in a team environment.

Doctor Of Philosophy - Phd, Molecular And Cell Biology

Master Of Science - Ms, Biochemistry

Bachelor Of Science - Bs, Chemistry (Honors)