A novel time-resolved luminescence assay system was developed, Peptide, protein, nanoparticle, ATP, GTP conjugates, peptide synthesis, and developed assay in the project.

Synthesis of Oligomannosides b-(1-2)-Linked by click chemistry, New Insights into Carbohydrate-Based Adjuvants in Immunotherapy, optimization and scale-up process,

Multi-step synthesis of API, route-scouting for drug candidates and process development, optimization, familiarization and scale up.During the early development and preparation of Clinical batches for Phase 1 and Phase 2. Synthesis of impurities and isolation of impurities by prep HPLC and interpretation NMR, Mass spectra analysis Design and development of synthetic routes, processes to the drug candidates based on application of technical knowledge by using an… Show more Multi-step synthesis of API, route-scouting for drug candidates and process development, optimization, familiarization and scale up.During the early development and preparation of Clinical batches for Phase 1 and Phase 2. Synthesis of impurities and isolation of impurities by prep HPLC and interpretation NMR, Mass spectra analysis Design and development of synthetic routes, processes to the drug candidates based on application of technical knowledge by using an appropriate resources, including on line journals and searching tools. Show less

Responsible : Multi-step synthesis, route-scouting for drug candidates and process development, optimization, familiarization and scale up.Synthesis of impurities and isolation of impurities by prep HPLC and interpretation NMR, UV, Mass spectra analysis. Technical transfer, kilo lab production of APIs, Documentation.

Synthesis and biochemical characterization of high-affinity inhibitors and luminescent probes for protein kinases. Supported by the results of X-ray analysis of inhibitor: kinase co-crystals and extensive structure-affinity studies enabled to constructed inhibitors whose affinity reached picomolar range. I was introduced to new methods for the synthesis of complicated heteroaromatic systems, bicycles, and tricycles. These aromatic fragments were conjugated with peptide analogs on solid phase… Show more Synthesis and biochemical characterization of high-affinity inhibitors and luminescent probes for protein kinases. Supported by the results of X-ray analysis of inhibitor: kinase co-crystals and extensive structure-affinity studies enabled to constructed inhibitors whose affinity reached picomolar range. I was introduced to new methods for the synthesis of complicated heteroaromatic systems, bicycles, and tricycles. These aromatic fragments were conjugated with peptide analogs on solid phase and labeled with fluorescent dyes. The obtained molecular conjugates possessed unique photoluminescent properties and they were used as optical probes for mapping of localization and monitoring activity of protein kinase in cells, but also HTS of inhibitors of protein kinases in biochemical binding/displacement assays. Further, he worked with photoluminescence (fluorescence and phosphorescence) based biochemical binding/displacement assays, using detection systems based on luminescence intensity, fluorescence polarization/anisotropy, time-gated luminescence intensity, and luminescence lifetime measurement. The aim of these measurements was the development of technologies for disease biomarker analysis. Show less