Roxanne Lourman Email & Phone Number

Zurich, Switzerland

Schlieren, Zurich, Suisse

Developed and optimized AAV production and characterization workflows from the ground-up, achieving a 10-fold increase in viral titers• Developed live-cell imaging assays to screen LNP libraries, improving insights into potency and efficiency• Developed multiplex immunoassays for LNP testing on whole blood and PBMCs to evaluate immunogenicity responses• Conducted >500 AAV productions at multiple scales for various AAV serotypes and scAAV • Screened rAAV construct libraries, optimizing sequence elements to boost potency, production, and puri-ty• Developed and validated advanced analytical methods, including ONT sequencing and multiplexed qPCR to assess viral genome integrity and DNA contaminants • Designed TaqMan and SYBR qPCR assays for titration of viral genomes and DNA contaminants• Operated under GDevP and GLP standards, meticulously documenting results in electronic laboratory notebook• Effectively communicated experimental results to internal and external audiences

Zürich Area, Switzerland

Led and contributed to research projects to improve disease modeling and pre-clinical testing for glioblastomasThesis: "The roles of MSH6 in tumor progression and radiotherapy response in glioblastomas"Major achievements: • Developed and characterized genetically engineered mouse models (GEMM) and conducted in vivo preclinical studies, including efficacy and PK/PD studies.• Developed advanced imaging workflows using QuPath and CellProfiler, integrating machine learning to study brain tumor microenvironments, immune infiltration, and therapeutic responses.• Created classifiers in QuPath for immune cell phenotyping and cell cycle assessment, offering insights into drug impact on immune modulation and cell cycle regulation.• Designed and analyzed multiplexed immunofluorescence assays with CellProfiler to assess DNA damage and repair pathways, identifying novel therapeutic targets for synthetic lethality.• Analyzed multi-omics and clinical data with R to identify genetic alterations and molecular mechanisms driving disease progression and therapy resistance.• Design and cloning of complex multi-component nucleic acid constructs• Generated stable KD and KO cell lines using RNAi and CRISPR/Cas9 gene editing• Developed > 10 novel molecular and cell-based assays to monitor DNA damage response and cell fate• Training and mentoring of 2 Master and 2 junior PhD students

Switzerland

Designed functional assays to support lead selection, target validation and mechanism of action studies, accelerating the company’s drug discovery pipeline• Led HTS of small molecule libraries using TR-FRET intracellular signaling assays for preclinical evaluation• Designed and optimized functional assays to validate lead binding and efficacy• Conducted ligand docking and homologous GPCR alignment to identify critical binding residues using PYMOL• Designed and conducted mutagenesis studies to confirm mechanism of action and allosteric modulation• Enhanced the sensitivity and reproducibility of drug screening assays, improving data quality

Région De Lausanne, Suisse

Purification and characterization of computer-designed small proteins for vaccine development and CAR T-cell therapy• Cloned > 50 expression vectors for protein production• Improved purification protocols to increase protein solubility and stability • Purified > 20 proteins, scFv fragments and antibodies using AKTA Pure purification systems• Led biophysical characterization of proteins (CD, MALS) and ligand-antibody binding affinity studies using SPR• Produced high-quality data that contributed to a peer-reviewed publication in a scientific journal

Peru

• Led the sponsorship and fundraising activities for an international development project, raising 10,000 CHF to build a nursery for 50 vulnerable children in Peru through negotiations with companies and events• Conducted interviews to understand local needs, preferences and constraints• Developed and managed the project plan and budget, demonstrating strong organizational skills and the ability to align resources effectively.• Coordinated a diverse team of 14 volunteers and local workers, fostering a collaborative environment that enhanced teamwork• Developed scale-up and sustainability plan to ensure long-term impact, enhancing my strategic thinking

Sevrier

Used my experience as a sailing competitor to teach sailing to groups of young children and adults, developing teaching skills, patience, and the ability to adapt instruction methods to various learning styles.

Annecy

Doctor Of Philosophy - Phd, Cancer Biology

Activities and Societies: PhD retreat organizationTitle : "The roles of MSH6 in tumor progression and radiotherapy response in glioblastoma"

Master'S Degree, Oncology And Cancer Biology, 5,5/6

Courses : • Cancer Biology (Advanced) • Immunology (Advanced) • Endocrinology • Stem Cell Technology • Project design in Translational.

Engineer'S Degree, Bioengineering And Biomedical Engineering, Excellent Mention, Ranking: 5Th

Activities and Societies: • Vice president of the music club: organizing concerts, managing the rehearsal schedule, controlling.

Bachelor Of Science - Bs, Preparatory Class, Mechanical Engineering, Excellent Mention

Ranked 5th Physic and Engineering preparatory class in France Intensive course to prepare for the competitive entrance exams.

Première Année Commune Des Études De Santé (Paces), Medicine, Ranked 350Th Out Of 1600 Students

Courses: • Cellular and molecular biology • Biochemistry and biophysics • Drug science and galenics • Histology • Immunology • Anatomy •.

High School Diploma, Science, General

Activities and Societies: Sailing competitionMathematics speciality