Robert Smith Email & Phone Number

Princeton, Nj

Developed facility for expression of proteins in liter scale (1-48L) generating lyophilized lysate as a deliverable. Mentored team which generated high throughput panel processes for a variety of outputs, including bacterial supernatants, purified proteins, and lyophilized product. Managed close client-supplier relationship, providing delivery metrics and updating scope and range of product offeringsEarned Lean Six Sigma Yellow Belt Accreditation (2021)

Princeton, New Jersey, United States

Developed protein purification team during creation of Princeton siteLed team in development of toolbox of endotoxin removal options for protein deliverables.

Malvern, Pa

Protein Scientist, Head of Protein ProductionManaged protein purification group which supplied reagents for services group andgenerates protein for external sales. Coordinated project management, time/resourcemanagement, and employee training/development. Established automation techniques to increase workflow, and defined strictQA/QC criteria for all products. Put into place new technology and workplaceefficiency changes. Tripled size and capacity of protein purification group, significantly expandingnumber of catalogue products offered yearly. Supervised development of multiple protein purification multi-column protocolsfor various HMTs, reader domains, and nucleosomes, with special emphasis onscalability and cost reduction.

Developed multiple protein purification multi-column protocols for various proteases, kinases, and other enzymes using a variety of AKTA platforms.Optimized and streamlined existing protocols for protein production to reduce timelines and increase efficiency of purificationScreened multiple variables to enhance protein expression, solubility, and stability including buffer conditions, detergents, additives, and construct lengthsRoutinely conducted biophysical characterization of proteins utilizing LC-MS, SEC, DLS, SLS, Thermofluor, and protein specific assay performance prior to protein distribution to other departments.

Dr. Paul Torrence'S Laboratory

Expressed and purified vaccinia virus thymidine kinase protein to 99% purity through anion HPLC, size exclusion HPLC, nickel affinity / ligand chromatographyPerformed Isothermal Calorimetry binding and kinetic experimentsTrained researchers and supervised undergraduate research projectsManaged laboratory facilities and up to 10 research staff on a daily operational basis

Dr. Judith Willis' Laboratory

Optimized expression and purification of AGCP2b proteinDeveloped database of cuticular proteins and performed extensive bioinformatics searches on cuticular proteins/DNA sequencesEstablished and streamlined RNA extraction and RT-PCR protocols Developed and optimized Differential Display protocol and subcloned multiple genes

Dr. Michael Terns Laboratory

Developed protein expression/purification protocols for fibrillarin proteinGeneration and characterization of polyclonal antibodies against fibrillarin proteinDeveloped protocols for transcribing/translating radiolabelled proteins in vivo

Master Of Business Administration - Mba, Business Administration, Strategic Leadership, Management And Operations

Ms, Chemistry

Bs, Genetics