Robert Stephenson Email & Phone Number

San Carlos, California, United States

Menlo Park, California, United States

• Developed procedures for the synthesis of antibody-oligonucleotide conjugates that increased yields from about 30% to over 70%.
• Established purification methods for conjugated antibodies on an AKTA Pure FPLC
• Developed QC methods based on SEC, RP and HIC HPLC chromatography.
• Wrote SOPs and successfully transferred methods to the manufacturing group.
• Manufactured conjugated antibodies for sale to support high customer demand for reagents.
• Received company team award in 2023 for contributions to the antibody conjugation project.

• Taught one semester of high school chemistry at Henry M. Gunn Senior High School.
• Taught two years of 7th and 8th grade science at Dartmouth Middle School.

Milpitas, California, United States

• Repaired dozens of different commercial assay kits used for the quantification of a wide variety of enzymes and metabolites.
• Optimized and improved ELISA assays targeting proteins and small molecules.
• Company Employee of the Year in 2018 for cost savings due to the repair of many valuable assay kit product lines.
• Ran Quality Control (QC) testing of metabolic and ELISA kits.
• Worked closely with research, manufacturing, QC, marketing and tech support to ensure product success.

South San Francisco, CA

• A key contributor to Trellis’ proprietary CellSpot technology for cloning human antibodies against specific disease targets.
• Established methods for isolating specific B cells of interest from large pools of leukocytes.
• Prepared antigen-coupled fluorescent microspheres for CellSpot screening.
• Measured antibody binding affinities using ForteBio Octet.
• Determined antibody thermal stabilities.

Palo Alto, CA

• Examined the effects of cell passage on human neural stem cells in an in vitro differentiation assay.
• Developed protocols for analysis of high-content image data from a cell-based differentiation assay.
• Produced and purified monoclonal antibodies for commercial sale.

• Established cell, protein, and gene-based assays for screening small molecule antifibrotics.
• Identified gene expression signatures for in vivo disease models of hypoxia, fibrosis, cancer, ischemic injury, and inflammation.
• Analyzed many large gene expression datasets.
• Responsible for designing and implementing methods for nucleic acid isolation, quantitative real-time PCR, and Affymetrix microarray studies.
• Co-authored 2 patents in the areas of fibrosis and anemia.

• Purification and biochemical characterization of a previously unknown protein identified by its homology to a phosphotriesterase.
• Developed an E. coli expression system for catalytic antibodies. Site-directed mutagenesis and characterization of changes in catalytic activity.

• Responsible for developing both binding and signal transduction assays for cloned human G protein-coupled receptors.
• Adapted a membrane capture-based receptor binding assay into a high-throughput screen.

• Screened a pool of synthetic DNA for individual molecules (aptamers) that bind tightly to polypeptides.

Ph.D., Biochemisty

· Identification and enzymology of a novel carboxyl methyltransferase using a lipidated synthetic peptide substrate. · In depth study of.

B.S., Chemistry