Laboratory Technician
Burlington, Vermont, United States
Trained mice and harvested neuronal tissue after cardiac perfusion.Transformed competent E Coli bacteria with various plasmids, containing desired DNA sequences; sustained bacterial lines, as well as grew/harvested resulting plasmids, which were submitted for in-house sequencingVerified proper DNA sequences using Invitrogens 'VECTOR NTI' software and BLAST searchesUtilized DNA sequences to identify appropriate restriction enzymes; linearized plasmids and performed run-off transcription using both biotin and digoxin labeled RNAHybridized synthetic labeled RNA to mouse tissue mRNAConducted immuno-histochemical labeling using DAB, alkaline-phosphatase, and TSA- fluorescent labeling systems .