Rohan Joshi Email & Phone Number

Dubai, Ae

United Arab Emirates

Senior Application Specialist

Dubai Media City, Dubai, United Arab Emirates

Duties and Responsibilities:Providing excellent customer service.Upselling to achieve monthly targets.Arranging daily nurses and driver schedule.Handling day to day operation using CRM i.e. AlayaCare, Salesforce, Sharpspring, Tawk.to.

Preston, United Kingdom

Preparing large quantities of E-Liquids.Volumetric measurement of the E-Liduids.Maintaining high quality and standards of the product.Keep up-to-date COSHH and Risk Assessment forms.

Preston, Uk

Optimise and use various Molecular and cell biologytechniques, data analysis, and liaise with clinical staff, orderlaboratory consumables and update COSHH and riskassessment, ethical issues. Supervisory assistance andprovided theoretical/ practical knowledge for BSc and MScprojects within the School.Training and demonstrating number of students in Cell Cultureand Molecular Biology laboratories. Monitoring and guidingstudents in sample preparations, Cell Culture, MediaPreparation, Cell Transfections, PCR sample preparation andPrimer Design.Invigilating and undertaking tutorial classes for undergraduatestudents and ensuring that the students followed properprotocols and adhered to COSHH and risk assessmentregulations.Transfer research skills to other undergraduate andpostgraduate students and contribute to other research projects,prepare and present posters, presentations. Fluent Skills set:qPCR.Primer designing.Restriction digestion.DNA Sequencing analysis.Polymerase Chain Reaction.Agarose Gel Electrophoresis.Cell Transfection utilising GFP protein.DNA/RNA extraction using magnetic particles.Spectro-analysis of DNA/RNA utilising Nanodrop machine.Cell Culture Techniques-Freezing/thawing and maintaining cell bank.Maintaining and sub-culturing various cell lines via routineculturing protocol (HEK293; U87MG, 1321N1 Glioblastoma celllines; SVGP12 human astrocytic cell line)Microbiological Techniques-Bacterial Cloning.Formaldehyde Fixation.Bacterial Plasmid extraction and purification.Primer designing with restriction site overhangs.Alkaline Phosphatase treatment for bacterial plasmid.Bacterial Transformation (Stbl3, NH10, Xl10, 10-beta E. Colistrains)Microscopy -Live Cell Imaging.Fluorescence Imaging.Bright field microscopy.Haemocytometer Cell counting.

Preston, United Kingdom

Picking up international students from airport.Assisting them in registering and enrolling to the university.Organizing campus and city tour and enrolling them to various ice-breaker sessions.Assisting new international students in finding accommodation and helping them settling in.