Rose Keating Email & Phone Number

Boston, Massachusetts, United States

Boston, Massachusetts, United States

● Established the viability of a viral protease sensor as a potential future alternative treatmentmethod for patients with chronic viral infection● Engineered RNA-editing enzymes based on adenosine deaminase acting on RNA (ADAR) tospecifically activate in response to viral protease presence● Executed mammalian cell culture and traditional cloning techniques● Analyzed system’s effectiveness via fluorescence microscopy, flow cytometry, and western blotanalysis●… Show more ● Established the viability of a viral protease sensor as a potential future alternative treatmentmethod for patients with chronic viral infection● Engineered RNA-editing enzymes based on adenosine deaminase acting on RNA (ADAR) tospecifically activate in response to viral protease presence● Executed mammalian cell culture and traditional cloning techniques● Analyzed system’s effectiveness via fluorescence microscopy, flow cytometry, and western blotanalysis● Achieved specific downstream target gene activity via protease activated engineered ADAR● Demonstrated system’s modularity and applicability by engineering ADAR with human-origincomponents to respond to the protease of a human pathogen Show less

Cambridge, Massachusetts, United States

● Lead fabricator on the BLOx project, which takes a microfluidic approach to artificial lungtechnology● Developed and improved BLOx fabrication procedures, constructed SOPs, organized and trackedfabrication schedule, tested device quality, executed ex vivo blood tests, trained new employeeson fabrication techniques● Supported increased BLOx device fabrication yield from 50% to 90%● Quantified BLOx device clotting after animal testing using ImageJ analysis to assess… Show more ● Lead fabricator on the BLOx project, which takes a microfluidic approach to artificial lungtechnology● Developed and improved BLOx fabrication procedures, constructed SOPs, organized and trackedfabrication schedule, tested device quality, executed ex vivo blood tests, trained new employeeson fabrication techniques● Supported increased BLOx device fabrication yield from 50% to 90%● Quantified BLOx device clotting after animal testing using ImageJ analysis to assess efficiency andblood health implications● Characterized the oxygen transfer efficiency of varying membrane materials and investigatedfabrication techniques to create a thin, uniform, oxygen transfer efficient membrane● Fabricated and tested combined artificial lung and kidney devices on the iMOS project● Implemented technical writing skills for the BLOx and iMOS projects Show less

Blacksburg, Virginia, United States

● Analyzed design matrices to determine how to isolate the biomarker miRNA through exosomes viadeterministic lateral displacement (DLD) for earlier detection of pancreatic cancer● Assessed DLD device improvements, modeled outputs, and optimized parameters for downstreamprototype fabrication and testing● Team leader responsible for organizing and hosting team meetings, ensuring the team completedgoals in a timely manner, and making executive decisions and final edits on… Show more ● Analyzed design matrices to determine how to isolate the biomarker miRNA through exosomes viadeterministic lateral displacement (DLD) for earlier detection of pancreatic cancer● Assessed DLD device improvements, modeled outputs, and optimized parameters for downstreamprototype fabrication and testing● Team leader responsible for organizing and hosting team meetings, ensuring the team completedgoals in a timely manner, and making executive decisions and final edits on submitted documentsand presentations Show less

Blacksburg, Virginia, United States

● Optimized a microfluidic device for buffer exchange of cells from a blood sample to a media that isoptimized for downstream analysis using contactless dielectrophoresis (cDEP)● Researched cell separation techniques, buffer components, electrical conductivity, and theaccuracy of the Ansys Fluent model’s parameters and outputs● Developed an introductory tutorial of Ansys Fluent for future undergraduate researchers andgained understanding of cDEP device operation and… Show more ● Optimized a microfluidic device for buffer exchange of cells from a blood sample to a media that isoptimized for downstream analysis using contactless dielectrophoresis (cDEP)● Researched cell separation techniques, buffer components, electrical conductivity, and theaccuracy of the Ansys Fluent model’s parameters and outputs● Developed an introductory tutorial of Ansys Fluent for future undergraduate researchers andgained understanding of cDEP device operation and fabrication process Show less

Master'S Degree, Biomedical Engineering

Biological Systems Engineering, Biotechnology