Roxanne Tymon Email & Phone Number

• Perform bioanalytical analysis to support early development of siRNA drugs to be used in treating rare diseases.
• Perform pharmacokinetic (PK) and pharmacodynamic (PD) analysis of rat and monkey plasma/serum and tissue samples that were dosed with siRNA.
• Execute techniques including RT-qPCR, RNA extraction, exosome extraction, OpenArray/mircroRNA profiling and ddPCR
• Design primers and probes.
• Complete immunoprecipitation assays to assess RISC loading of siRNA.

Waltham, MA

• Performed antibody/antigen purification of new targets to be used as antibody drug conjugates (ADCs) for cancer research. Supported Discovery and Biochemistry groups by providing high-quality purified material within.
• Performed purifications at varying scales using AKTA-FPLC and characterized using SEC-HPLC. - Purification skills included protein A, Ceramic Hydroxyapatite (CHT), Q/SP-sepharose and immobilized metal chromatography..
• Ran developability assessment of lead antibody candidates through stability studies and SDS-PAGE analysis.
• Applied multiple techniques including the kinetic chromogenic LAL assay testing for endotoxin, DNA purification at mini and maxi scales, and transient transfections (HEK293T-S and ExpiCHO cells).
• Presented results at group meetings and maintained an electronic laboratory notebook (ELN).

Waltham, MA

Waltham, MA

• Investigated gene regulation of the Nucleosome Remodeling Factor (NURF) in response to cellular stress. The functional role of the NURF subunits under copper stress was examined through dsRNAi experiments. Confirmed.
• Applied techniques including preparation of dsRNA, RNAi in Drosophila cells, cDNA synthesis, qPCR, western blotting, cell culture, gel electrophoresis, and PCR.

Worcester, MA

• Examined protein interaction partners and regulation of MIG-10, an adaptor protein involved in neuronal migration and axon guidance during development in C. elegans. Revealed a key association between MIG-10 and CSN-5.
• Executed techniques including gene cloning via Gateway Cloning System, co-immunoprecipitation, western blotting, gel electrophoresis, PCR, and cell culture.

Falmouth, MA

Performed whole blood specimen collection and preparation from Atlantic Horseshoe Crabs, Limulus Polyphemus. Isolated whole blood constituents using sterile techniques for use in endotoxin and glucan testing of injectable medications. Lab managerial duties including record keeping, glassware preparation and general lab maintenance.

Master'S Degree, Molecular And Cell Biology

Bachelor Of Science (Bs), Biology And Biotechnology

Minored in Spanish. Completed an Interactive Qualifying Project Abroad in Melbourne, Australia. Completed a Major Qualifying Project.