Ryan Bringhurst Email & Phone Number

Greater Salt Lake City Area

Sandy, UT

Tucson, Arizona

• Managing a $400,000 annual budget.
• Leading a lab of 3 research technicians and 2 undergraduates.
• Used immunofluorescence and Western blotting to demonstrate that glutamine deprivation causes reactivation of HSV-1 and two other herpesviruses. This work is the basis for a clinical trial currently being conducted at.
• Examined gene expression with luciferase and gfp expression assays.
• Used an ELISA to examine cellular DNA synthesis and a slot… Show more
• Used an ELISA to examine cellular DNA synthesis and a slot blot hybridization assay to examine viral DNA synthesis.

Greater Boston Area

• Used flow cytometry to examine the relationship between the cell cycle and replication an HSV-1 mutant. The results contradicted a 1991 paper that was widely accepted in the field.
• Used virus plaque assays and growth curves to determine how cellular stress affects virus replication.
• Identified through microarray analysis cellular genes whose expression is affected by cellular stresses that cause enhanced plating efficiency of an HSV-1 mutant virus.
• Used flow cytometry and… Show more
• Used flow cytometry and Western blots to demonstrate that glutamine deprivation causes reactivation of two herpesviruses.
• Used a mouse ocular model followed by neuronal explantation and cell culture to study HSV-1 reactivation following glutamine deprivation.

Storrs, Connecticut

• Studied the nodulating and nitrogen-fixing bacterium Sinorhizobium meliloti and its symbiotic partner alfalfa for Ph.D. thesis.
• Generated more than 100 unique vectors using gene cloning methods and site-directed mutagenesis.
• Made more than 100 unique bacterial strains by high throughput random mutagenesis and by directed mutagenesis using integration of suicide vectors.
• Conducted high throughput transposon mutagenesis and analyzed mutants by inverse PCR followed by sequencing… Show more
• Conducted high throughput transposon mutagenesis and analyzed mutants by inverse PCR followed by sequencing and BLAST searches.
• Confirmed directed mutations by Southern blotting.

Rockville, Maryland

• Purified polysaccharide-protein conjugates by size exclusion chromatography (SEC).
• Developed conjugate vaccines consisting of a polysaccharide backbone with cross-linked protein. These vaccines are now in production.
• Anesthetized and inoculated cotton rats.
• Investigated immunologic response of cotton rat animal model to respiratory syncytial virus (RSV).

Ph.D., Microbiology

B.A., Chemistry

A.S.