Ryan Mcmullin Email & Phone Number

Cambridge, Massachusetts, United States

Waltham, Massachusetts, United States

Greater Boston Area

Diagnostic Lead for Immuno-Oncology: Women's Cancers and Novel Combinations Global Product TeamEarly Oncology Portfolio (preclinical to Ph2) Diagnostic lead, multiple Innovative Medicine Teams

Spring House, Pa

Department Of Pathology

Discovery and Development of Prognostic and Predictive Biomarkers for Breast Cancer and Pairing with Targeted Pharmaceuticals - Laboratory/project management (team of 2-3 technologists/technicians)- Data analysis and interpretation to derive biological states, context, and biomarkers- Gene expression analyses (Taqman qRT-PCR, microarray, Nanostring, RNA-Seq) of archival (FFPE, Frozen) clinical specimens (whole, laser capture microdissected)- Assays for in vitro pharmacological and metabolic evaluation (colorimetric, fluorometric, familiarity with NMR spectroscopy through collaboration)- Lentiviral/retroviral transduction, chemical transfection, and Nucleofection for manipulation of gene expression (siRNA, shRNA, ZFNs, TALENs, constitutive and inducible overexpression) in mammalian cell culture (2D, 3D)- Protein expression, localization, interaction, and activation state evaluation and mapping (Immunoblotting, Immunohistochemistry, immunofluorescence, Co-Immunoprecipitation, familiarity with RPMA and LC-MS/MS through collaborations)- Microscopy (light, fluorescent, confocal)- Murine transgenic/knockout husbandry and evaluation (mammary gland development and oncogenesis)- Primary manuscript, grant, and IRB protocol writing experience

Department Of Pathology

- Concurrent academic appointment

Development of Mouse Models that Recapitulate Key Pathological Features of Prostate Adenocarcinoma and Genomic Analysis of Transcriptional Regulation - Extensive cloning and genetic engineering of mouse and human genome sequence using restriction enzymes and homologous recombination techniques (yeast and bacterial) for the generation of transgene constructs in plasmids, cosmids, and BACs- Screening, breeding, and maintenance of murine transgenic lines- Analysis of in vivo transgene expression in all tissue (primary focus on prostate and colorectum) throughout anterior-posterior development, organogenesis, and adult homeostasis, prostate tumorigenesis, and metastatic outgrowth - Tissue processing (paraffin embedding, sectioning)- Tissue staining/examination (H&E, Immunohistochemistry)- Gene expression analyses (Northern blot, SYBR-green Q-PCR) - Protein and Antibody purification (Ni and protein affinity chromatography)- Bioinformatic analyses of genomes for regulatory element identification (VISTA Tools, Genomatix software)- Chromatin immunoprecipitation and analysis- Primary manuscript writing

- Managed client accounts and billed for service- Prepared and processed samples for sequencing- Provided consultation on data interpretation and troubleshooting- Performed maintenance on the Applied Biosystems Prism 310Genetic Analyzer- Ordered and maintained laboratory equipment and consumables

Doctor Of Philosophy (Phd), Molecular And Cell Biology

Master Of Science (M.S.), Applied Molecular Biology